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Genetic Methods for Cellular Manipulations in C. elegans

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C. elegans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1327))

Abstract

Neuron manipulation in vivo by ablation, activation, or inactivation, and regulation of gene expression, is essential for dissecting nervous system function. Here we describe genetic means for neuron manipulation in the nematode C. elegans, and provide protocols for generating transgenic animals containing these genetic tools.

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Acknowledgment

I would like to thank Shai Shaham, Aakanksha Singhvi, In Hae Lee, and Sean Wallace for comments and discussions on this manuscript.

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Authors and Affiliations

  1. Laboratory of Developmental Genetics, The Rockefeller University, 810 Weiss Research Building, 1230 York Avenue, New York, NY, 10065, USA

    Menachem Katz

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  1. Menachem Katz
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Correspondence to Menachem Katz .

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Editors and Affiliations

  1. Department of Physics, The James Franck Institution, The Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA

    David Biron

  2. Federated Department of Biological Sciences, New Jersey Institute of Technology and Rutgers University, Newark, New Jersey, USA

    Gal Haspel

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Katz, M. (2015). Genetic Methods for Cellular Manipulations in C. elegans . In: Biron, D., Haspel, G. (eds) C. elegans. Methods in Molecular Biology, vol 1327. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4939-2842-2_3

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  • DOI: https://doi.org/10.1007/978-1-4939-2842-2_3

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4939-2841-5

  • Online ISBN: 978-1-4939-2842-2

  • eBook Packages: Springer Protocols

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