Skip to main content

Stabilization and Controlled Release of Invertase Through the Addition of Trehalose in Wet and Dried Alginate-Chitosan Beads

  • Chapter
Water Stress in Biological, Chemical, Pharmaceutical and Food Systems

Part of the book series: Food Engineering Series ((FSES))

Abstract

The stability and controlled release of certain active substances (such as flavors, drugs, enzymes, essential oils, etc.) can be achieved through encapsulation. Ionically cross-linked hydrogels such as alginate- or alginate-chitosan-CaCl2 beads were proposed as suitable materials for encapsulation systems (Deladino et al. 2008; Han et al. 2008). Alginate beads coated with chitosan were used for encapsulation and release of different proteins (Coppi and Iannuccelli 2009; Zhou et al. 2010). Direct interaction between them forms beads with improved mechanical properties associated with reinforcement of bead structure due to chitosan binding to free alginate sites by cooperative ionic bounds (Deladino et al. 2008).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Abbreviations

A:

Alginate

A ¥ :

Maximum invertase activity corresponding to the active invertase present in the beads

A t :

Invertase activity at time t

A-TCh:

Alginate-trehalose-chitosan beads

Ch:

Chitosan

FD:

Freeze-drying

k :

Constant related to geometric and structural characteristics to the macromolecular network

n :

Release mechanism type

SEM:

Scanning electron microscopy

T :

α-α-Trehalose dehydrate

t :

Time

VD:

Vacuum drying

References

  • Alfrey E, Gurnee EF, Lloyd WG (1966) Diffusion in glassy polymers. J Polym Sci C 12:249–261

    Article  Google Scholar 

  • Bernfeld P (1955) Amylases, α and β. Methods Enzymol 1:149–158

    Article  CAS  Google Scholar 

  • Coppi G, Iannuccelli V (2009) Alginate/chitosan microparticles for tamoxifen delivery to the lymphatic system. Int J Pharm 367:127–132

    Article  CAS  Google Scholar 

  • Deladino L, Anbinder PS, Navarro AS, Martino MN (2008) Encapsulation of natural antioxidants extracted from Ilex paraguariensis. Carbohydr Polym 71:126–134

    Article  CAS  Google Scholar 

  • Wee SF, Gombotz WR (1998) Protein release from alginate matrices. Adv Drug Deliv Rev 31:267–285

    Article  Google Scholar 

  • Han J, Guenier A-S, Salmieri S, Lacroix M (2008) Alginate and chitosan functionalization for micronutrient encapsulation. J Agric Food Chem 56:2528–2535

    Article  CAS  Google Scholar 

  • Lee SL, Hafeman AE, Debenedetti PG, Pethica BA, Moore DJ (2006) Solid-state stabilization of α-chymotrypsin and catalase with carbohydrates. Ind Eng Chem Res 45:5134–5147

    Article  CAS  Google Scholar 

  • Martinsen A, Skjak-Braek G, Smidsrød O (1989) Alginate as immobilization material: I. Correlation between chemical and physical properties of alginate gel beads. Biotechnol Bioeng 33:79–89

    Article  CAS  Google Scholar 

  • Mi F-L, Her N-L, Kuan C-Y, Wong T-B, Shyu S-S (1997) Chitosan tablets for controlled-release of theophylline—effect of polymer-drug wet or dry blending and anionic-cationic interpolymer complex. J Appl Polym Sci 66:2495–2505

    Article  CAS  Google Scholar 

  • Peppas NA (1985) Analysis of Fickian and non-Fickian drug release from polymers. Pharm Acta Helv 60:110–111

    CAS  Google Scholar 

  • Pothakamury UR, Barbosa-Cánovas GV (1995) Fundamental aspects of controlled release in foods. Trends Food Sci Technol 6:397–406

    Article  CAS  Google Scholar 

  • Santagapita PR, Gómez Brizuela L, Mazzobre MF, Ramirez H, Corti HR, Villalonga Santana R, Buera MP (2008) Structure/function relationships of several biopolymers as related to invertase stability in dehydrated systems. Biomacromolecules 9:741–747

    Article  CAS  Google Scholar 

  • Santagapita PR, Mazzobre MF, Buera MP (2011) Formulation and drying of alginate beads for controlled release and stabilization of invertase. Biomacromolecules 12:3147–3155

    CAS  Google Scholar 

  • Santagapita PR, Mazzobre MF, Buera MP (2012) Invertase stability in alginate beads: effect of trehalose and chitosan inclusion and of drying methods. Food Res Int 47:321–330

    Article  CAS  Google Scholar 

  • Schülke N, Schmid FX (1988) Effect of glycosylation on the mechanism of renaturation of invertase from yeast. J Biol Chem 263:8832–8837

    Google Scholar 

  • Shu S, Sun C, Zhang X, Wu Z, Wang Z, Li C (2010) Hallow and degradable polyelectrolyte nanocapsules for protein drug delivery. Acta Biomater 6:210–217

    Article  CAS  Google Scholar 

  • Smidsrød O, Skjak-Braek G (1990) Alginate as immobilization matrix for cells. Trends Biotechnol 8:71–78

    Article  Google Scholar 

  • Zhou Z-d, Li G-y, Li Y-j (2010) Immobilization of Saccharomyces cerevisiae alcohol dehydrogenase on hybrid alginate-chitosan beads. Int J Biol Macromol 47:21–26

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors acknowledge the financial support of ANPCYT (PICT 0928), CONICET (PIP 100846), and UBA (Project UBACyT 20020100100397).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. P. Buera .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media New York

About this chapter

Cite this chapter

Santagapita, P.R., Mazzobre, M.F., Buera, M.P. (2015). Stabilization and Controlled Release of Invertase Through the Addition of Trehalose in Wet and Dried Alginate-Chitosan Beads. In: Gutiérrez-López, G., Alamilla-Beltrán, L., del Pilar Buera, M., Welti-Chanes, J., Parada-Arias, E., Barbosa-Cánovas, G. (eds) Water Stress in Biological, Chemical, Pharmaceutical and Food Systems. Food Engineering Series. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2578-0_28

Download citation

Publish with us

Policies and ethics