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Agrobacterium Protocols pp 245–257Cite as

Citrus Transformation Using Juvenile Tissue Explants

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1224))

Abstract

The most frequently used method for production of citrus transgenic plants is via Agrobacterium-mediated transformation of tissues found on explants obtained from juvenile seedlings. Within the last decade and especially within the last 5–6 years, this robust method was employed to produce thousands of transgenic plants. With the newly applied screening methods that allow easier and faster detection of transgenic shoots, estimates of transformation rate for some cultivars have gone up making this approach even more attractive. Although adjustments have to be made regarding the (varietal) source of the starting material and Agrobacterium strain used in each experiment preformed, the major steps of this procedure have not changed significantly if at all. Transgenic citrus plants produced this way belong to cultivars of rootstocks, sweet oranges, grapefruits, mandarins, limes, and lemons.

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Author information

Authors and Affiliations

  1. Citrus Research and Education Center, University of Florida/IFAS, 700 Experiment Station Road, Room 103, Lake Alfred, FL, 33850, USA

    Vladimir Orbović

  2. Citrus Research and Education Center, University of Florida/IFAS, Lake Alfred, FL, 33850, USA

    Jude W. Grosser

Authors
  1. Vladimir Orbović
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  2. Jude W. Grosser
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Corresponding author

Correspondence to Vladimir Orbović .

Editor information

Editors and Affiliations

  1. Plant Sciences Institute, and Department of Agronomy, Center for Plant Transformation, Iowa State University, Ames, Iowa, USA

    Kan Wang

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Orbović, V., Grosser, J.W. (2015). Citrus Transformation Using Juvenile Tissue Explants. In: Wang, K. (eds) Agrobacterium Protocols. Methods in Molecular Biology, vol 1224. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1658-0_20

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  • DOI: https://doi.org/10.1007/978-1-4939-1658-0_20

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-1657-3

  • Online ISBN: 978-1-4939-1658-0

  • eBook Packages: Springer Protocols

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