Abstract
Function of lectin depends on its amino acid sequence of carbohydrate-recognition domain (CRD), conformation, and extracellular/intracellular localization. Altering lectin gene expression by over-expression or knockdown is a powerful tool for analyzing its cellular function. Here, we describe a method of lectin gene over-expression, taking a C-type lectin, mannan-binding protein (MBP), as an example. Carbohydrate-binding ability of MBP, its subcellular localization, and functional co-localization with ligand glycoprotein are assayed comparing with an inactive mutant MBP.
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Nonaka, M., Kawasaki, T. (2014). Functional Assay Using Lectin Gene Targeting Technologies (Over-Expression). In: Hirabayashi, J. (eds) Lectins. Methods in Molecular Biology, vol 1200. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1292-6_34
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DOI: https://doi.org/10.1007/978-1-4939-1292-6_34
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1291-9
Online ISBN: 978-1-4939-1292-6
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