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Plastid Biology pp 517–557Cite as

Synthesis of Recombinant Products in the Chloroplast

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Part of the book series: Advances in Plant Biology ((AIPB,volume 5))

Abstract

For the engineering of new traits in plants and algae, modification of the plastid genome offers significant advantages. These include transgene integration by homologous recombination, high levels of expression, multigene engineering in polycistronic units, and reduced transmission through pollen. Numerous tools are available for DNA delivery, selection of transformants, removal of undesirable markers and efficient expression of single or multiple transgenes. The list of plants and algae with successful plastid transformation is increasing and includes some crop plants. Its further extension to agronomically relevant species and appropriate cultivars, together with advances in synthetic biology, will be key to the future development of plastids as green factories for the production of high-value metabolites or proteins.

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Abbreviations

3′UTR:

3′-Untranslated region

5′UTR:

5′-Untranslated region

AHAS:

Acetohydroxyacid synthase

Bt :

Bacillus thuringiensis

CES:

Control by epistasy of synthesis

CTB:

Cholera toxin B

DB:

Downstream box

GOI:

Gene of interest

GRAS:

Generally recognized as safe

HPPD:

Hydroxyphenyl pyruvate dioxygenase

HSA:

Human serum albumin

IEE:

Intercistronic expression element

IFT:

Isoxaflutole

IR:

Inverse repeat regions of the plastome

LHRR:

Left homologous recombination region

NEP:

Nucleus-encoded RNA polymerase

NTRC:

NADPH-dependent thioredoxin reductase C

PEG:

Polyethylene glycol

PEP:

Plastid-encoded RNA polymerase

RBPs:

RNA-Binding-Proteins

RHRR:

Right homologous recombination region

TSP:

Total soluble protein

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Acknowledgements

The authors thank Nicolas Roggli for preparing figures. SP acknowledges support for work on algal transplastomics from the UK Biotechnology and Biological Sciences Research Council (grant BB/I007660/1). Work in the laboratory of MGC was supported by the University of Geneva and by grants from the Swiss NRP59 programme (Benefits and Risks of the Deliberate Release of Genetically Modified Plants), from the European Union FP7 KBBE 2009-3 Sunbiopath (GA 245070) and from the Swiss National Science Foundation (3100A-117712).

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Authors and Affiliations

  1. Alganelle, 813 Chemin de Pingon, 73290, La Motte-Servolex, France

    Ghislaine Tissot-Lecuelle

  2. Institute of Structural and Molecular Biology, University College London, Gower Street, WC1E 6BT, London, UK

    Saul Purton

  3. Bayer CropScience, 3500 Paramount Parkway, 27560, Morrisville, NC, USA

    Manuel Dubald

  4. University of Geneva, 30 quai E. Ansermet, 1211, Geneva 4, Switzerland

    Michel Goldschmidt-Clermont

Authors
  1. Ghislaine Tissot-Lecuelle
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  2. Saul Purton
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  3. Manuel Dubald
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  4. Michel Goldschmidt-Clermont
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Corresponding author

Correspondence to Michel Goldschmidt-Clermont .

Editor information

Editors and Affiliations

  1. Department of Plant Biology, Univeristy of California-Davis, Davis, California, USA

    Steven M. Theg

  2. Physiologie Membranaire et Moléculaire du Chloroplaste, Institut de Biologie Physico-Chimique, Paris, France

    Francis-André Wollman

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© 2014 Springer Science+Business Media New York

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Tissot-Lecuelle, G., Purton, S., Dubald, M., Goldschmidt-Clermont, M. (2014). Synthesis of Recombinant Products in the Chloroplast. In: Theg, S., Wollman, FA. (eds) Plastid Biology. Advances in Plant Biology, vol 5. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-1136-3_18

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