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Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells

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Hematopoietic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1185))

Abstract

Hematopoietic stem and progenitor cells (HSPCs) reside mainly in bone marrow; however, under homeostatic and stressed conditions, HSPCs dynamically change their location—either egressing from bone marrow and getting into circulation, a process of mobilization; or coming back to the bone marrow, the homing process. How to analyze these two processes will be critical for understanding the behavior of HSPCs. Here we provide an experimental protocol to monitor and analyze homing and migration of HSPCs.

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References

  1. Sugimura R, He XC, Venkatraman A et al (2012) Noncanonical Wnt signaling maintains hematopoietic stem cells in the niche. Cell 150:351–365

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Acknowledgements

This work was supported by Stowers Institute for Medical Research.

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Authors and Affiliations

  1. Stowers Institute for Medical Research, Kansas City, MO, USA

    Xi C. He, Rio Sugimura, Meng Zhao & Linheng Li

  2. Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA

    Zhenrui Li

  3. Sanford Consortium for Regenerative Medicine, University of California, San Diego La Jolla, CA, USA

    Jason Ross

  4. Stowers Institute for Medical Research, 1000 East 50th Street, 64110, Kansas City, MO, USA

    Linheng Li

Authors
  1. Xi C. He
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  2. Zhenrui Li
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  3. Rio Sugimura
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  4. Jason Ross
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  5. Meng Zhao
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  6. Linheng Li
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Corresponding author

Correspondence to Linheng Li .

Editor information

Editors and Affiliations

  1. Department of Pediatrics, Aflac Cancer and Blood Disorders Center Emory University School of Medicine, Atlanta, Georgia, USA

    Kevin D. Bunting

  2. Department of Pediatrics, Emory University School of Medicine Aflac Cancer & Blood Disorders Ctr, Atlanta, Georgia, USA

    Cheng-Kui Qu

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© 2014 Springer Science+Business Media New York

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He, X.C., Li, Z., Sugimura, R., Ross, J., Zhao, M., Li, L. (2014). Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells. In: Bunting, K., Qu, CK. (eds) Hematopoietic Stem Cell Protocols. Methods in Molecular Biology, vol 1185. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1133-2_19

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  • DOI: https://doi.org/10.1007/978-1-4939-1133-2_19

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1132-5

  • Online ISBN: 978-1-4939-1133-2

  • eBook Packages: Springer Protocols

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