Abstract
Listeria monocytogenes, a foodborne intracellular pathogen, is responsible for about 300 deaths every year in the USA. It has the ability to escape host defense mechanisms and causes listeriosis more frequently in immunocompromised individuals. Virulence mechanisms in L. monocytogenes are highly regulated and tightly controlled. A number of virulence factors that play important roles in pathogenesis of listeriosis have been identified and characterized. This review highlights the power of comparative genomics and functional genomics in identifying genes and proteins involved in the infection process. These genes and proteins are potentially useful as biomarkers for detecting virulent L. monocytogenes. This review also focuses on developments in the in vivo and in vitro models used in characterization of listerial virulence.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Barton Behravesh C, Jones TF, Vugia DJ, Long C, Marcus R, Smith K, Thomas S, Zansky S, Fullerton KE, Henao OL, Scallan E (2011) Deaths associated with bacterial pathogens transmitted commonly through food: foodborne diseases active surveillance network (FoodNet), 1996–2005. J Infect Dis 204:263–267
Hamon M, Bierne H, Cossart P (2006) Listeria monocytogenes: a multifaceted model. Nat Rev Microbiol 4:423–434
Seeliger HP (1984) Modern taxonomy of the Listeria group relationship to its pathogenicity. Clin Invest Med 7:217–221
Barbour AH, Rampling A, Hormaeche CE (2001) Variation in the infectivity of Listeria monocytogenes isolates following intragastric inoculation of mice. Infect Immun 69:4657–4660
Kim SH, Bakko MK, Knowles D, Borucki MK (2004) Oral inoculation of A/J mice for detection of invasiveness differences between Listeria monocytogenes epidemic and environmental strains. Infect Immun 72:4318–4321
Roche SM, Gracieux P, Albert I, Gouali M, Jacquet C, Martin PM, Velge P (2003) Experimental validation of low virulence in field strains of Listeria monocytogenes. Infect Immun 71:3429–3436
Liu D, Ainsworth AJ, Austin FW, Lawrence ML (2003) Characterization of virulent and avirulent Listeria monocytogenes strains by PCR amplification of putative transcriptional regulator and internalin genes. J Med Microbiol 52:1065–1070
Lecuit M, Cossart P (2002) Genetically-modified-animal models for human infections: the Listeria paradigm. Trends Mol Med 8:537–542
Lecuit M, Sonnenburg JL, Cossart P, Gordon JI (2007) Functional genomic studies of the intestinal response to a foodborne enteropathogen in a humanized gnotobiotic mouse model. J Biol Chem 282:15065–15072
Mengaud J, Ohayon H, Gounon P, Mege RM, Cossart P (1996) E-cadherin is the receptor for internalin, a surface protein required for entry of L. monocytogenes into epithelial cells. Cell 84:923–932
Lecuit M, Dramsi S, Gottardi C, Fedor-Chaiken M, Gumbiner B, Cossart P (1999) A single amino acid in E-cadherin responsible for host specificity towards the human pathogen Listeria monocytogenes. EMBO J 18:3956–3963
Lecuit M, Vandormael-Pournin S, Lefort J, Huerre M, Gounon P, Dupuy C, Babinet C, Cossart P (2001) A transgenic model for listeriosis: role of internalin in crossing the intestinal barrier. Science 292:1722–1725
Levraud JP, Disson O, Kissa K, Bonne I, Cossart P, Herbomel P, Lecuit M (2009) Real-time observation of Listeria monocytogenes-phagocyte interactions in living zebrafish larvae. Infect Immun 77:3651–3660
Coombes JL, Robey EA (2010) Dynamic imaging of host-pathogen interactions in vivo. Nat Rev Immunol 10:353–364
Chambers MC, Song KH, Schneider DS (2012) Listeria monocytogenes infection causes metabolic shifts in Drosophila melanogaster. PLoS One 7:e50679
Guha S, Klees M, Wang X, Li J, Dong Y, Cao M (2013) Influence of planktonic and sessile Listeria monocytogenes on Caenorhabditis elegans. Arch Microbiol 195:19–26
Joyce SA, Gahan CG (2010) Molecular pathogenesis of Listeria monocytogenes in the alternative model host Galleria mellonella. Microbiology 156:3456–3468
Bouwer HG, Bai A, Forman J, Gregory SH, Wing EJ, Barry RA, Hinrichs DJ (1998) Listeria monocytogenes-infected hepatocytes are targets of major histocompatibility complex class Ib-restricted antilisterial cytotoxic T lymphocytes. Infect Immun 66:2814–2817
Temm-Grove CJ, Jockusch BM, Rohde M, Niebuhr K, Chakraborty T, Wehland J (1994) Exploitation of microfilament proteins by Listeria monocytogenes: microvillus-like composition of the comet tails and vectorial spreading in polarized epithelial sheets. J Cell Sci 107(Pt 10):2951–2960
Bierne H, Travier L, Mahlakoiv T, Tailleux L, Subtil A, Lebreton A, Paliwal A, Gicquel B, Staeheli P, Lecuit M, Cossart P (2012) Activation of type III interferon genes by pathogenic bacteria in infected epithelial cells and mouse placenta. PLoS One 7:e39080
Yamada F, Ueda F, Ochiai Y, Mochizuki M, Shoji H, Ogawa-Goto K, Sata T, Ogasawara K, Fujima A, Hondo R (2006) Invasion assay of Listeria monocytogenes using Vero and Caco-2 cells. J Microbiol Methods 66:96–103
Popov A, Driesen J, Abdullah Z, Wickenhauser C, Beyer M, Debey-Pascher S, Saric T, Kummer S, Takikawa O, Domann E, Chakraborty T, Kronke M, Utermohlen O, Schultze JL (2008) Infection of myeloid dendritic cells with Listeria monocytogenes leads to the suppression of T cell function by multiple inhibitory mechanisms. J Immunol 181:4976–4988
Gaillard JL, Berche P, Mounier J, Richard S, Sansonetti P (1987) In vitro model of penetration and intracellular growth of Listeria monocytogenes in the human enterocyte-like cell line Caco-2. Infect Immun 55:2822–2829
Jaradat ZW, Bhunia AK (2003) Adhesion, invasion, and translocation characteristics of Listeria monocytogenes serotypes in Caco-2 cell and mouse models. Appl Environ Microbiol 69:3640–3645
Glaser P, Frangeul L, Buchrieser C, Rusniok C, Amend A, Baquero F, Berche P, Bloecker H, Brandt P, Chakraborty T, Charbit A, Chetouani F, Couve E, de Daruvar A, Dehoux P, Domann E, Dominguez-Bernal G, Duchaud E, Durant L, Dussurget O, Entian KD, Fsihi H, Garcia-del Portillo F, Garrido P, Gautier L, Goebel W, Gomez-Lopez N, Hain T, Hauf J, Jackson D, Jones LM, Kaerst U, Kreft J, Kuhn M, Kunst F, Kurapkat G, Madueno E, Maitournam A, Vicente JM, Ng E, Nedjari H, Nordsiek G, Novella S, de Pablos B, Perez-Diaz JC, Purcell R, Remmel B, Rose M, Schlueter T, Simoes N, Tierrez A, Vazquez-Boland JA, Voss H, Wehland J, Cossart P (2001) Comparative genomics of Listeria species. Science 294:849–852
den Bakker HC, Cummings CA, Ferreira V, Vatta P, Orsi RH, Degoricija L, Barker M, Petrauskene O, Furtado MR, Wiedmann M (2010) Comparative genomics of the bacterial genus Listeria: genome evolution is characterized by limited gene acquisition and limited gene loss. BMC Genomics 11:688
Buchrieser C, Rusniok C, Garrido P, Hain T, Scortti M, Lampidis R, Karst U, Chakraborty T, Cossart P, Kreft J, Vazquez-Boland JA, Goebel W, Glaser P (2011) Complete genome sequence of the animal pathogen Listeria ivanovii, which provides insights into host specificities and evolution of the genus Listeria. J Bacteriol 193:6787–6788
Hain T, Ghai R, Billion A, Kuenne CT, Steinweg C, Izar B, Mohamed W, Mraheil MA, Domann E, Schaffrath S, Karst U, Goesmann A, Oehm S, Puhler A, Merkl R, Vorwerk S, Glaser P, Garrido P, Rusniok C, Buchrieser C, Goebel W, Chakraborty T (2012) Comparative genomics and transcriptomics of lineages I, II, and III strains of Listeria monocytogenes. BMC Genomics 13:144
Doumith M, Buchrieser C, Glaser P, Jacquet C, Martin P (2004) Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J Clin Microbiol 42:3819–3822
Liu D, Lawrence ML, Austin FW, Ainsworth AJ (2007) A multiplex PCR for species- and virulence-specific determination of Listeria monocytogenes. J Microbiol Methods 71:133–140
Chatterjee SS, Hossain H, Otten S, Kuenne C, Kuchmina K, Machata S, Domann E, Chakraborty T, Hain T (2006) Intracellular gene expression profile of Listeria monocytogenes. Infect Immun 74:1323–1338
Joseph B, Przybilla K, Stuhler C, Schauer K, Slaghuis J, Fuchs TM, Goebel W (2006) Identification of Listeria monocytogenes genes contributing to intracellular replication by expression profiling and mutant screening. J Bacteriol 188:556–568
Camejo A, Buchrieser C, Couve E, Carvalho F, Reis O, Ferreira P, Sousa S, Cossart P, Cabanes D (2009) In vivo transcriptional profiling of Listeria monocytogenes and mutagenesis identify new virulence factors involved in infection. PLoS Pathog 5:e1000449
Van de Velde S, Delaive E, Dieu M, Carryn S, Van Bambeke F, Devreese B, Raes M, Tulkens PM (2009) Isolation and 2-D-DIGE proteomic analysis of intracellular and extracellular forms of Listeria monocytogenes. Proteomics 9:5484–5496
Hain T, Hossain H, Chatterjee SS, Machata S, Volk U, Wagner S, Brors B, Haas S, Kuenne CT, Billion A, Otten S, Pane-Farre J, Engelmann S, Chakraborty T (2008) Temporal transcriptomic analysis of the Listeria monocytogenes EGD-e sigmaB regulon. BMC Microbiol 8:20
Raengpradub S, Wiedmann M, Boor KJ (2008) Comparative analysis of the sigma B-dependent stress responses in Listeria monocytogenes and Listeria innocua strains exposed to selected stress conditions. Appl Environ Microbiol 74:158–171
Arous S, Buchrieser C, Folio P, Glaser P, Namane A, Hebraud M, Hechard Y (2004) Global analysis of gene expression in an rpoN mutant of Listeria monocytogenes. Microbiology 150:1581–1590
Donaldson JR, Nanduri B, Pittman JR, Givaruangsawat S, Burgess SC, Lawrence ML (2011) Proteomic expression profiles of virulent and avirulent strains of Listeria monocytogenes isolated from macrophages. J Proteomics 74:1906–1917
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Reddy, S., Lawrence, M.L. (2014). Virulence Characterization of Listeria monocytogenes . In: Jordan, K., Fox, E., Wagner, M. (eds) Listeria monocytogenes. Methods in Molecular Biology, vol 1157. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0703-8_13
Download citation
DOI: https://doi.org/10.1007/978-1-4939-0703-8_13
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0702-1
Online ISBN: 978-1-4939-0703-8
eBook Packages: Springer Protocols