Abstract
Compared with luciferase which is widely used as a reporter for circadian rhythms in Arabidopsis thaliana, available fluorescent markers are generally too stable to allow circadian oscillations to be measured. However, we have developed a technique to use the nuclear localization of circadian-controlled transcription factors fused to a fluorescent reporter as a means of measuring circadian rhythms. This technique has the advantage of being suitable for analyzing rhythms at the level of individual cells and in living plants.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Nagel DH, Kay SA (2012) Complexity in the wiring and regulation of plant circadian networks. Curr Biol 22:648–657
James AB, Monreal JA, Nimmo GA, Kelly CL, Herzyk P, Jenkins GI, Nimmo HG (2008) The circadian clock in Arabidopsis roots is a simplified slave version of the clock in shoots. Science 322:1832–1835
Para A, Farre EM, Imaizumi T, Pruneda-Paz JL, Harmon FG, Kay SA (2007) PRR3 is a vascular regulator of TOC1 stability in the Arabidopsis circadian clock. Plant Cell 19:3462–3473
Yakir E, Hilman D, Kron I, Hassidim M, Melamed-Book N, Green RM (2009) Posttranslational regulation of CIRCADIAN CLOCK ASSOCIATED1 in the circadian oscillator of Arabidopsis. Plant Physiol 150:844–857
Davis AM, Hall A, Millar AJ, Darrah C, Davis SJ (2009) Protocol: streamlined sub-protocols for floral-dip transformation and selection of transformants in Arabidopsis thaliana. Plant Methods 5:3
Acknowledgement
This work was supported by US Friends of Hebrew University grant 0367445, ISF grant 0398636 and DFG grant 0308300.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Shor, E., Hassidim, M., Green, R.M. (2014). The Use of Fluorescent Proteins to Analyze Circadian Rhythms. In: Staiger, D. (eds) Plant Circadian Networks. Methods in Molecular Biology, vol 1158. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0700-7_14
Download citation
DOI: https://doi.org/10.1007/978-1-4939-0700-7_14
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0699-4
Online ISBN: 978-1-4939-0700-7
eBook Packages: Springer Protocols