Abstract
The interaction between the cytotoxic T lymphocyte (CTL) and its target cell has been a model of immune cellular interaction for several years because of the relatively short time course between the actual receptor-ligand interaction and its functional expression in target cell lysis. The nature of the lethal event within the target remains unclear but recent experiments from our laboratory and others have suggested that CTL mediated lysis involves not only the production of an osmotic lesion in the plasma membrane but also rapid changes in nuclear and chromatin structure (see ref. #1 for review). This nuclear lesion distinguishes CTL mediated lysis from cell death mediated by Ab+C1 or hypotonic shock.
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References
Russell, J.H. 1983. Internal disintegration model of cytotoxic lymphocyte-induced target damage. Immunol. Rev. 72:97.
Golstein, P. and E.T. Smith. 1977. Mechanisms of T-cell mediated cytolysis: the lethal hit stage. Contemp. Top. Immunol. 4:273.
Martz, E. 1977. Mechanism of specific tumor cell lysis by alloimmune T lymphocytes: resolution and characterization of discrete steps in the cellular interaction. Contemp. Top. Immunol. 4:301.
Russell, J.H. and C.B. Dobos. 1980. Mechanisms of immune lysis. II. CTL-induced nuclear disintegration of the target begins within minutes of cell contact. J. Immunol. 125:1256.
Reinherz, E.L., S. C., Meuer and S.F. Schlossman. 1983. The human T cell receptor: analysis with cytotoxic T cell clones. Immunol. Rev. 74:83.
Martz, E., W. Heagy and S.H. Gromkowski. 1983. The mechanism of CTL-mediated killing: monoclonal antibody analysis of the roles of killer and target-cell membrane proteins. Immunol. Rev. 72:73.
Tsoukas, C.D., P.A. Carson, S. Fong and J.H. Vaughan. 1982. Molecular interactions in human T cell-mediated cytotoxicity to EBV. II. Monoclonal antibody 0KT3 inhibits a post-killer-target recognition/adhesion step. J. Immunol. 129:1421.
Harris, D.T., H.R., MacDonald and J.-C., Cerottin. 1984. Direct transfer of antigen specific cytolytic activity to noncytolytic cells upon fusion with liposomes derived from cytolytic T cell clones. J. Exp. Med. 159:261.
Dennert, G. and E. Podack. 1983. Cytolysis by H-2 specific T killer cells: Assembly of tubular complexes or target membranes. J. Exp. Med. 157:1483.
Glasebrook, A.L., M. Sarmiento, M.R. Loken, et al., 1980. Murine T lymphocyte clones with distinct immunological functions. Immunol. Rev. 51:93.
Howe, R.C. and J.H. Russell. 1983. Isolation of alloreactive CTL clones with cyclical changes in lytic activity. J. Immunol. 131:2141.
Russell, J.H. and C.B. Dobos. 1983. Characterization of a “heteroclitic” cytotoxic lymphocyte clone: heterogeneity of receptors or signals? J. Immunol. 130:538.
Bonavida, B., T.P. Bradley and E.A. Grimon. 1983. Frequency determination of killer cells by a single-cell cytotoxic assay. Meth. in Enzymology. 93:270.
Russell, J.H. 1984. Phorbol esters inactivate the lytic apparatus of cytotoxic T lymphocytes. J. Immunol, (in press).
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© 1985 Plenum Press, New York
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Russell, J.H., Howe, R.C. (1985). Evidence for the Molecular Dissociation of Binding and Post-Binding Functions in Cytotoxic T Lymphocytes. In: Henkart, P., Martz, E. (eds) Mechanisms of Cell-Mediated Cytotoxicity II. Advances in Experimental Medicine and Biology, vol 184. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-8326-0_30
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DOI: https://doi.org/10.1007/978-1-4684-8326-0_30
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