Summary
A four-step purification system using high performance liquid chromatography (HPLC) was developed and used in conjunction with a rapid and sensitive bioassay to isolate and purify a number of myotropic peptides present in methanolic head extracts of the cockroach, Leucophaea maderae, and the house cricket, Aceta domesticus. The extracts were sequentially fractionated on a series of four HPLC columns exhibiting different separation characteristics. The procedure yielded peptides of sufficient purity for primary sequence determination. A total of 17 unique peptides, including the first gastrin/cholecystokinin-like invertebrate peptides, were structurally characterized and synthesized.
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© 1990 Plenum Press, New York
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Holman, G.M., Nachman, R.J., Wright, M.S. (1990). A Strategy for the Isolation and Structural Characterization of Certain Insect Myotropic Peptides that Modify the Spontaneous Contractions of the Isolated Cockroach Hindgut. In: McCaffery, A.R., Wilson, I.D. (eds) Chromatography and Isolation of Insect Hormones and Pheromones. Chromatographic Society Symposium Series. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-8062-7_18
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DOI: https://doi.org/10.1007/978-1-4684-8062-7_18
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