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NMR Studies of the Enzyme Mechanisms of Vitamin B12 Biosynthesis

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Bioorganic Chemistry in Healthcare and Technology

Part of the book series: NATO ASI Series ((NSSA,volume 207))

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Abstract

For the past 20 years our laboratory has been engaged in the elucidation of the vitamin B12 biosynthetic pathway.1. By 1977 it had been established that 5-aminolevulinic acid (ALA), porphobilinogen (PBG) uro’gen III and three intermediates, Factors I-III, now known to be transformed as the precorrins 1–3, were sequentially formed on the way to the corrin nucleus as summarized in Scheme 1. The ensuing decade has witnessed an exponential increase in the rate of the acquisition of pure enzymes using recombinant DNA techniques and these methods together with the exploration of new NMR pulse sequences have not only cast light on the mechanisms of the B12 synthetic enzymes but have given us considerable optimism with regard to the discovery of the remaining intermediates and of the enzymes which produce them.

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Authors and Affiliations

  1. Center for Biological NMR, Department of Chemistry, Texas A&M University, College Station, Texas, 77843-3255, USA

    A. Ian Scott

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  1. A. Ian Scott
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Editors and Affiliations

  1. University of Amsterdam, Amsterdam, The Netherlands

    Upendra K. Pandit

  2. University of Antwerp (RUCA), Antwerp, Belgium

    Frank C. Alderweireldt

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© 1991 Plenum Press, New York

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Scott, A.I. (1991). NMR Studies of the Enzyme Mechanisms of Vitamin B12 Biosynthesis. In: Pandit, U.K., Alderweireldt, F.C. (eds) Bioorganic Chemistry in Healthcare and Technology. NATO ASI Series, vol 207. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1354-0_14

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  • DOI: https://doi.org/10.1007/978-1-4684-1354-0_14

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