Abstract
Plasma cholesterol level Is a risk factor for the development of coronary artery disease (1). Indeed, the cholesterol bound to low-density lipoprotein (LDL) appears to better correlate with the risk of coronary heart disease (CHD) as compared to plasma cholesterol levels while the cholesterol bound to high-density lipoprotein (HDL) is inversely related to the risk of developing CHD.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Goldstein JL, Brown MS. Familial hypercholesterolemia. In Stanbury JB, Wyngaarden JB, Fredrickson DS, Goldstein JL, Brown MS, eds. The metabolic basis of inherited disease. New York, 672–712 (1983).
Avogaro P, Bittolo Bon G, Cazzolato G, Quinci GB, Belussi F. Plasma level of lipoprotein A-I and apolipoprotein B in human atherosclerosis. Artery 4, 385–394 (1978).
Riesen WF, Mordasini R, Salzmann C, Theler A., Gurtner HP. Apoproteins and lipids as discriminators of severity of coronary heart disease. Atherosclerosis 37, 157–162 (1980).
De Backer G, Rosseneu M, Deslypere JP. Discriminative value of lipids and apoproteins in coronary heart disease. Atherosclerosis 42, 197–203 (1982).
Köhler G, Milstein C. Continuous cultures of fused cells secreting antibodies of predifined specificity. Nature 256, 495–497 (1975).
Tsao BP, Curtiss LK, Edgington TS. Immunochemical heterogeneity of human plasma apolipoprotein B. II. Expression of apolipoprotein B epitopes on native lipoproteins. J Biol Chem 257, 15222–15228 (1982).
Marcovina S, France D, Phillips RA, Mao SJT. Monoclonal antibodies can precipitate low-density lipoprotein. I. Characterization and use in determining apolipoprotein B. Clin Chem 31, 1654–1658 (1985).
Curtiss LK, Edgington TS. Immunochemical heterogeneity of human plasma high density lipoproteins. Identification with apolipoprotein A-I and A-II specific monoclonal antibodies. J Biol Chem 260, 2982–2993 (1985).
Marcovina S, Kottke BA, Mao SJT. Monoclonal antibodies can precipitate low density lipoprotein. II. Radioimmunoassay with single and combined monoclonal antibodies for determining apolipoprotein B in serum of patients with coronary artery disease. Clin Chem 31, 1659–1663 (1985).
Marcovina S, Di Cola G, Rapetto C, et al. Development of a radial immunodiffusion technique employing monoclonal antibodies for apolipoprotein B determination in human plasma. Clin Chim Acta 147, 117–125 (1985).
Marcovina S, Price PJ, McDougal JS, Cremonesi L. Monoclonal antibodies directed against human low density lipoprotein and their characterization by the immunodot technique. Protides Biological Fluids Proc Colloq 31, 985–989 (1984).
Scanu AM, Edelstein C. Solubility in aqueous solutions of ethanol of the small molecular weight peptides of the serum very low density and high density lipoproteins. Relevance to the recovery problem during delipidation of serum lipoproteins. Analyt Biochem 53, 576–588 (1973).
Egusa G, Brady DW, Grundy SM, Howard BV. Isopropanol precipitation method for the determination of apolipoprotein B specific activity and plasma concentration during metabolic studies of very low density lipoprotein apolipoprotein B. J Lip Res 24, 1261–1267 (1983).
Markwell MAK, Haas SM, Bieber LL, Toebert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 87, 206–210 (1978).
Wayne TF, Alaupovic P, Curry MD, et al. Plasma apolipoprotein B and VLDL-, LDL-, and HDL-cholesterol as risk factors in the development of coronary artery disease in male patients examined by angiography. Atherosclerosis 39, 411–424 (1981).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Plenum Press, New York
About this chapter
Cite this chapter
Marcovina, S., Catapano, A.L. (1987). Use of Combined Monoclonal Antibodies for the Immunochemical Determination of Apolipoproteins A–I and B in Human Plasma. In: Malmendier, C.L., Alaupovic, P. (eds) Lipoproteins and Atherosclerosis. Advances in Experimental Medicine and Biology, vol 210. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1268-0_10
Download citation
DOI: https://doi.org/10.1007/978-1-4684-1268-0_10
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-1270-3
Online ISBN: 978-1-4684-1268-0
eBook Packages: Springer Book Archive