Summary
Thylakoid biogenesis in Chlamydomonas reinhardtii y-1 occurred at a linear rate when degreened cells incubated at 38°C were exposed to light. Photosystem 2 (PS2) activity, which was negligible in degreened cells, increased in parallel with chlorophyll (Chl). Kinetic parameters suggest that these PS2 units were larger and more efficient in light capture than those made at 25° C. Membranes made during the initial minutes of greening emanated from the chloroplast envelope. Kinetics of accumulation of chlorophyll a/b-binding (Cab) proteins implied that these proteins were degraded immediately upon import into the plastid unless Chl was available. Molecular modeling studies suggest that Chl is required to convert these proteins from an extended, protease-sensitive conformation into a compact, folded complex. A protease was partially purified that may be involved in degradation of the uncomplexed Cab proteins. These studies support the hypothesis that assembly of thylakoid membranes in this organism occurs in association with the chloroplast envelope.
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© 1992 Springer Science+Business Media New York
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Hoober, J.K., Marks, D.B., Gabriel, J.L., Paavola, L.G. (1992). Role of the Chloroplast Envelope in Thylakoid Biogenesis. In: Argyroudi-Akoyunoglou, J.H. (eds) Regulation of Chloroplast Biogenesis. Nato ASI Series, vol 226. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3366-5_45
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DOI: https://doi.org/10.1007/978-1-4615-3366-5_45
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