Abstract
The vascular endothelium represents an important interface between the blood vessel lumen and the surrounding tissues, and regulates vasotonicity, permeability, homeostasis and angiogenesis. The adhesive interactions between endothelial cells (EC) and their surrounding extracellular matrix (ECM) regulate growth, differentiation and migration1. The localization of endothelial cells at the contact with leukocytes lead to their exposure to peroxides during local inflammatory reactions. It has been reported on that many inflammatory mediators, including reactive oxygen species (ROS) and cytokines modulate EC-leukocyte interaction,2 In contrast little reports have focused on EC-ECM interactions. The purpose of this study was to set-up an in-vitro model for studying the effects of oxidative stress on EC-ECM interactions, using two immortalized cell lines derived from human umbilical vein cord (HUVEC), and classified as endothelial. ECV304 spontaneously transformed HUVEC,3 and EA.hy926 a hybridoma of the epithelial cell line A549 and HUVEC,4 were used to study the effects of ter-Butyl Hydroperoxide (t-BHP) on cell adhesion to collagen I, intracellular glutathione (GSH) depletion, ROS production, integrin expression and cytoskeleton organization.
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References
E. Ruoslahti and E. Engvall. Integrins and vascular extracellular matrix assembly. J Clin Invest. 99:1149 (1997).
J.R. Bradley, D. Johnson, and J.S. Pober. Endothelial activation by hydrogen peroxide: selective increase of intracellular adhesion molecule-1 and major histocompatibility complex class I. Am J Pathol 142:1598 (1993).
K. Takahashi, Y. Sawasaki, J. Hata, K. Mukai, and T. Goto. Spontaneous transformation and immortalization of human endothelial cells in vitro. Cell Dev Biol. 26:265 (1990).
C.J. Edgell, C.C. McDonald, and J.B. Graham. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc Natl Acad USA. 80:3734 (1983)
F. Braut-Boucher, J. Pichon, P. Rat, M. Adolphe, M. Aubery, and J. Font. Anon isotopic, highly sensitive fluorimetric, cell-cell adhesion microplate assay using calcein AM-labeled lymphocytes. J Immunol Meth. 178:41 (1995).
S. Rappeneau, A. Baeza-Squiban, F. Braut-Boucher, M. Aubery, M.C. Gendron, F.Marano. Use of fluorescent probes to assess the early sulfhydryl depletion and oxidative stress induced by mechlorethamine in human bronchial epithelial cells. Toxicol in vitro. 13:765 (1999)
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© 2001 Springer Science+Business Media New York
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Lamari, F. et al. (2001). Opposite Effects of Oxidative Stress on Endothelial Cell Lines (ECV 304 and Eahy 926) Interaction With Extracellular Matrix. In: Dansette, P.M., et al. Biological Reactive Intermediates VI. Advances in Experimental Medicine and Biology, vol 500. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0667-6_37
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DOI: https://doi.org/10.1007/978-1-4615-0667-6_37
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5185-6
Online ISBN: 978-1-4615-0667-6
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