Abstract
Successful tumor sterilization by Photodynamic Therapy (PDT) depends on delivery of a light dose appropriate for the concentration of photoactive HPD in the tumor at the time of irradiation. Since the concentration of HPD in target cells or tissues is an important determinant of response to PDT, we have radioactively labelled HPD with the positron emitter Copper-64 to allow non-invasive in-vivo quantitation of HPD concentration by external nuclear detectors. Imaging of HPD distribution is also made possible by Positron Emission Tomography (PET). Labelling with the pure gamma-emitting Copper-67 would permit standard nuclear scintigraphy or Single Photon Emmission Computed Tomography (SPECT) in patients, the longer half-life of 67Cu (58 hours compared to 13 hours for 64Cu) being advantageous in this application.
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References
Altman, K.I., Casarett, G.W., Masters, R.E., Noonan, T.R., and Salomon, K., 1948, Hemoglobin synthesis from glycine labelled with radioactive carbon in its α-carbon atom. J. Biol. Chem., 176: 319–325.
Andreoni, A., De Silvestri, S., Laporta, P., Jori, G., and Reddi, E., 1982, Hematoporphyrin derivative: experimental evidence for aggregated species, Chem. Phys. Lett., 88: 33–36.
Borsook, H., Graybiel, A., Keighley, G., and Windsor, E., 1954, Polycythemic response in normal adult rats to a nonprotein plasma extract from anemic rabbits, Blood, 9: 734–742.
Brown, S.B., and Shillcock, M., 1976, Equilibrium and kinetic studies of the aggregation of porphyrins in aqueous solution, Biochem. J., 153: 279–284.
Christensen, T., Moan, J., Sandquist, T., and Smedshammer, L., 1984, Multicellular spheroids as an in vitro model system for photoradiation therapy in the presence of HPD, in: “Porphyrin Localization and Treatment of Tumors”, D. Doiron, and C. Gomer, eds., Alan R. Liss, Inc., New York: 381–390.
Falk, J.E., 1964, “Porphyrins and Metalloporphyrins. Elsevier Publishing”, Amsterdam.
Firnau, G., Maass, G., Wilson, B.C., and Jeeves, W.P., 1984, 64Cu labelling of hemataporphyrin derivative for non-invasive in-vivo measurements of tumour uptake, in: “Porphyrin Localization and Treatment of Tumors”, loc cit: 629–636.
Gomer, C.J., and Dougherty, T.J., 1979, Determination of 3H- and 14C- hemataporphyrin derivative distribution in malignant and normal tissue, Cancer Res. 39: 146–151.
Gomer, C.J., Rucker, N., Mark, C., Benedict, W.F., and Murphree, A.L., 1982, Tissue distribution of 3H-hematoporphyrin derivative in athymic “nude” mice heterotransplanted with human retinoblastoma, Invest. Opthalmol. Vis. Sci., 22: 118–120.
Labbe, R.F., and Nishida, G., 1957, A new method of hemin isolation, Biochim. Biophys. Acta, 26: 437.
Lipson, R.L., Baldes, E.J., and Olsen, A.M., 1961, The use of a derivative of hematoporphyrin in tumor detection, J. Natl. Cancer Inst., 26: 1–8.
Kessel, D., 1985. Personal communication.
Kessel, D., and Cheng, M.L., 1985, Studies on the biological and biophysical properties of dihematoporhyrin ether, the tumour-localizing component of HPD, Cancer Res., in Press.
Sutherland, R.M., and Durand, R.E., 1976, Radiation response of multicell spheroids - an in-vitro tumour model, Current Topics in Radiation Res., 11: 87–139.
Wharen, R.E., Anderson, R.E., and Laws, E.R., 1983, Quantitation of hematoporphyrin derivative in human gliomas, experimental central nervous system tumors, and normal tissues, Neurosurgery, 12: 446–450.
Williams, J.F., 1973, Oncogenic transformation of hamster embryo cells in-vitro by adenovirus type 5, Nature, 243: 162–163.
Wise, B., and Taxdal, D.R., 1967, Studies of the blood-brain barrier utilizing hematoporphyrin, Brain Research, 4: 387–389.
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© 1985 Plenum Press, New York
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Jeeves, W.P., Wilson, B.C., Firnau, G., Brown, K. (1985). Studies of Hpd and Radiolabelled Hpd In-Vivo and In-Vitro . In: Kessel, D. (eds) Methods in Porphyrin Photosensitization. Advances in Experimental Medicine and Biology, vol 193. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2165-1_7
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DOI: https://doi.org/10.1007/978-1-4613-2165-1_7
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