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Effects of Ultrahigh Vacuum and UV Irradiation on Transforming DNA of Haemophilus Influenzae

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Terrestrial Space Radiation and Its Biological Effects

Part of the book series: Nato ASI Series ((NSSA,volume 154))

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Abstract

Dry transforming DNA of Haemophilus influenzae UV-irradiated under 1 bar and vacuum, respectively, exhibits an increased UV-resistance of the Sm-marker as compared to DNA irradiated in solution. The photoreactivation sector of dried DNA was zero. These investigations are performed as preparatory experiments to the Eureca experiment “Exobiological Unit” ER161.

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References

  • Bücker, H., and Horneck, G., 1969, Untersuchungen über die wirkung von Vakuum und Strahlung auf Escherichia coli, Biophysik, 6:68.

    Article  Google Scholar 

  • Bücker, H., Horneck, G., and Wollenhaupt, H., 1971, Inactivation and division delay of Escherichia coli B/r by combined treatment with UV and vacuum, Biophysik, 7:217.

    Article  PubMed  Google Scholar 

  • Bücker, H., Dose, K., Horneck, G., and Thomas, C., 1979, A special photoproduct of UV-irradiated DNA in vacuo, Life Sciences and Space Research, XVII:111.

    Google Scholar 

  • Harm, W., 1980, “Biological effects of ultraviolet radiation”, Cambridge University Press, Cambridge.

    Google Scholar 

  • Herriott, R. M., Meyer, E. Y., Vogt, M., and Modan, M., 1970, Defined medium for growth of Haemophilus influenzae, J. Bacteriol., 101:513.

    PubMed  CAS  Google Scholar 

  • Horneck, G., and Bücker, H., 1971, Reparatur von UV-Schaden nach Bestrahlung von Bakterien im Vakuum, Strahlentherapie, 141: 723.

    Google Scholar 

  • Horneck, G., Bücker, H., Dose, K., Martens, K. D., Mennigmann, H. D., Reitz, G., Requardt, H., and Weber, P., 1984a, Photobiologie in space: An experiment on Spacelab I, Origins of Life, 14:825.

    Article  CAS  Google Scholar 

  • Horneck, G., Bücker, H., Reitz, G., Requardt, H., Dose, K., Martens, K. D., Mennigmann, H. D., and Weber, P., 1984b, Microorganisms in the space environment, Science, 225:226.

    Article  CAS  Google Scholar 

  • Kaplan, R. W., 1955, Mutation und Tbtung durch UV in Bakterien verschiedenen wassergehalts, Naturwissenschaften, 7:184.

    Article  Google Scholar 

  • Marmur, J., 1961, A procedure for the isolation of deoxyribonucleic acid from micro-organisms, J. Mol. Biol., 3:208.

    Article  CAS  Google Scholar 

  • Pons, F. W., and Mennigmann, H. D., 1973, Acetone-sensitized photoinactivation of transforming DNA, Photochem. Photobiol., 18:17.

    Article  PubMed  CAS  Google Scholar 

  • Rahn, O. R., and Hosszu, J. L., 1968a, Photochemistry of polynucleotides: A summery of temperature effects, Photochem. Photobiol., 7:637.

    Article  CAS  Google Scholar 

  • Rahn, O. R., and Hosszu, J. L., 1968b, Photoproduct formation in DNA at low temperatures, Photochem. Photobiol., 8:53.

    Article  CAS  Google Scholar 

  • Rahn, R. O., and Hosszu, J. L., 1969, Influence of relative humidity on the photochemistry of DNA films, Biochim. Biophys. Acta, 190:126.

    PubMed  CAS  Google Scholar 

  • Smith, K. C., and Yoshikawa, H., 1966, Variation in the photochemical reactivity of thymine in DNA of B.subtilis spores, vegetative cells and spores germinated in chloramphenicol, Photochem. Photobiol., 5:777.

    Article  PubMed  CAS  Google Scholar 

  • Webb., S. J., 1972, Semi-dehydration and the action of ultraviolet light, in,“Research progress in organic, biological medicinal chemistry”, Vol. 3, Part II, U. Gallo, and 382 L. Santamaria, (Eds.), North-Holland Publishing Company, Amsterdam.

    Google Scholar 

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© 1988 Plenum Press, New York

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Kiss, K., Mennigmann, HD. (1988). Effects of Ultrahigh Vacuum and UV Irradiation on Transforming DNA of Haemophilus Influenzae. In: McCormack, P.D., Swenberg, C.E., Bücker, H. (eds) Terrestrial Space Radiation and Its Biological Effects. Nato ASI Series, vol 154. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1567-4_28

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  • DOI: https://doi.org/10.1007/978-1-4613-1567-4_28

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8859-6

  • Online ISBN: 978-1-4613-1567-4

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