Abstract
The isolation of peptides through the use of monoclonal antibody immunoadsorbent columns was developed. The α chain of cholera toxin was used as a model for application of this strategy. Monoclonal antibodies to the A subunit of cholera toxin were isolated from mouse hybridoma cell lines. Two monoclonal antibodies with different specificities were used to immunoadsorb a cyanogen bromide digest of either purified native toxin or the α chain of the toxin. The amino-terminal sequences of the isolated fragments were determined by automated sequence analysis. In addition, a S. aureus protease digest was immunoadsorbed with one of the monoclonal antibodies.
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References
Zabin, I., Fowler, A.V., and Brake, A.J. (1977). In Solid Phase Methods in Protein Sequence Analysis, Previero, A. and Colleti-Previero, M.A. eds., North- Holland Publishing Co., New York, pp. 257 – 263.
Finkelstein, R.A. (1973). CRC Crit. Rev. Microbiol. 2, 553 – 623.
LoSpalluto, J.J., and Finkelstein, R.A. (1972). Biochem. Biophys. Acta 257, 158 – 166.
Duffy, L.K., Peterson, J.W., and Kurosky, A. (1981) FEBS Lett., 126, 187–190.
Lai, C.-Y. (1977). J. Biol. Chem. 252, 7249 – 7256.
Kurosky, A., Markel, D.E., and Peterson, J.W. (1977). J. Biol. Chem. 252, 5257 – 5264.
Duffy, L.K., Peterson, J.W., and Kurosky, A. (1981). J. Biol. Chem., in press.
Lai, C.-Y. (1980). CRC Crit. Rev. Biochem. 9, 171–206.
Finkelstein, R.A. (1975). In Current Topics in Microbiology and Immunology, Vol. 69, Springer-Verlag, New York, pp. 137 – 195.
Markel, D.E., Hejtmancik, K.E., Peterson, J.W., and Kurosky, A. (1979). J. Supramolec. Struct. 10, 137 – 149.
Kurosky, A., Duffy, L.K., and Denney, R.M. (1981). In Protides of Biological Fluids, Peeters ed., Pergamon Press, Oxford, Vol. 29, in press.
Bureau, D., and Daussant, J. (1981). J. Immunol. Meth. 41, 387 – 392.
Caldwell, H.D., and Kuo, C.C. (1977). J. Immunol. 118, 437 – 441.
Lai, C.-Y., Cancedda, F., and Chang, D. (1979). FEBS Lett. 100, 85–89.
Schroeder, W.A., Shelton, J.B., and Shelton, J.R. (1980). Hemoglobin 4, 551–559.
Mahoney, N.C., and Hermodson, M.A. (1980). J. Biol. Chem. 255, 11199 – 11203.
Springer, T.A. (1981). J. Biol. Chem. 256, 3833 – 3839.
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© 1982 The HUMANA Press Inc.
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Duffy, L.K., Kurosky, A. (1982). The Application of Monoclonal Antibodies to the Microsequencing of Proteins. In: Elzinga, M. (eds) Methods in Protein Sequence Analysis. Experimental Biology and Medicine, vol 3. Humana Press. https://doi.org/10.1007/978-1-4612-5832-2_11
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DOI: https://doi.org/10.1007/978-1-4612-5832-2_11
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