Abstract
Microscopy tools for direct visualization of cytological details include the light microscope, the confocal scanning microscope, and the transmission electron microscope (TEM). The scanning electron microscope involves circuitry that processes electronic Signals into an indirect image seen on a cathode ray tube or digital screen. All of the direct visualization methods involve light or electrons interacting with specimens and utilize either glass or magnetic lenses to project an image to the eye, to a screen, or to film. When considering the development of electron microscopes, it is useful to recognize the antecedents from the realm of light microscopy. The concepts of electron optics underlying electron microscopes are primarily extrapolations from the physics of light optics.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Agar, A.W. 1957. On the screen brightness required for high resolution Operation of the electron microscope. Br. J. Appl. Phys. 8: 410.
Agar, A.W. 1974. Operation of the electron microscope. In: A.W. Agar, R.H. Anderson, and D. Chescoe (eds.), Principles and practice of electron microscope Operation (pp. 166–190). North-Holland, Amsterdam.
Beeston, B.E.P., Horne, R.W., and Markham, R. 1972. Electron diffraction and optical diffraction techniques. In: A.M. Gauert (ed.), Practical methods in electron microscopy (Vol. 1). North-Holland, Amsterdam.
Ghadially, EN. 1985. Diagnostic electron microscopy of tumors, 2nd edn. Butterworths, London.
Grubb, D.T., and Keller, A. 1972. Beam induced radiation damage in polymers and its effect on the image formed in the electron microscope. Proc. 5th Eur. Conf. Electron Microsc., Manchester, p. 554.
Haguenau, F., Hawkes, P.W., Hutchison, J.L., Satiat-Jeunemaitre, B., Simon, G.T., and Williams, D.B. 2003. Key events in the history of election microscopy. Microsc. Microanal. 9:96.
Hainfeld, J.F. 1977. Understanding and using field emission sources. Scan. Electron Microsc. 1: 591.
Hawkes, P.W. (ed.). 1985. The beginnings of electron microscopy. Academic Press, New York.
Knoll, M., and Ruska, E. 1932. The electron microscope. 2. Physik. 78: 318.
Meek, G.A. 1976. Practical electron microscopy for biologists, 2nd edn. John Wiley & Sons, New York.
Robinson, D.G., Ehlers, IL, Herken, R., Herrmann, B., Mayer, F., and Schurmann, F.-W., 1987. Methods of preparation for electron microscopy. Springer-Verlag, Berlin.
Slayter, E.M. 1976. Optical methods in biology. Robert E. Krieger, Huntington, New York.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2003 Michael J. Dykstra
About this chapter
Cite this chapter
Dykstra, M.J., Reuss, L.E. (2003). Transmission Electron Microscopy. In: Biological Electron Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9244-4_17
Download citation
DOI: https://doi.org/10.1007/978-1-4419-9244-4_17
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-4856-6
Online ISBN: 978-1-4419-9244-4
eBook Packages: Springer Book Archive