Abstract
Electrostatics role is studied in protein adsorption to phosphatidylcholine (PC) and PC/phosphatidylglycerol (PG) small unilamellar vesicles (SUVs). Protein interaction is monitored vs. PG content at low ionic strength. Adsorption of lysozyme, myoglobin and bovine serum albumin (BSA) isoelectric point (pI) is investigated in SUVs, along with changes in protein fluorescence emission spectra. Partition coefficients and cooperativity parameters are calculated. At pI, binding is maximum while at lower/higher pHs binding drops. In Gouy–Chapman model activity coefficient goes with square charge number, which deviations indicate asymmetric location of anionic lipid in the bilayer inner leaflet, in agreement with experiments and molecular dynamics simulations. Vesicles bind myoglobin anti-cooperatively and lysozyme/BSA cooperatively. Hill coefficient reflects subunit cooperativity of bi/tridomain proteins.
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References
Torrens F, Campos A, Abad C (2003) Binding of vinyl polymers to anionic model membranes. Cell Mol Biol 49:991–998
Torrens F, Abad C, Codoñer A, García-Lopera R, Campos A (2005) Interaction of polyelectrolytes with oppositely charged micelles studied by fluorescence and liquid chromatography. Eur Polym J 41:1439–1452
Torrens F, Castellano G, Campos A, Abad C (2007) Negatively cooperative binding of melittin to neutral phospholipid vesicles. J Mol Struct 834–836:216–228
Torrens F, Castellano G, Campos A, Abad C (2009) Binding of water-soluble, globular proteins to anionic model membranes. J Mol Struct 924–926:274–284
Torrens F, Castellano G. (2009) Comparative analysis of the electrostatics of the binding of cationic proteins to vesicles: Asymmetric location of anionic phospholipids. Anal Chim Acta 654:2–10
Pérez-Gil J, Keough KMW (1998) Interfacial properties of surfactant proteins. Biochim Biophys Acta 1408:203–217
Torrens F, Castellano G (2006) Periodic classification of local anaesthetics (procaine analogues). Int J Mol Sci 7:12–34
Sánchez-Muñoz OL, Nordström EG, Prérez-Hernández E (2003) Electrophoretic and thermodynamic properties for biomaterial particles with bovine serum albumin adsorption. Biomed Mater Eng 13:147–158
Acknowledgements
The authors dedicate this manuscript to Prof. Dr Agustín Campos, who was greatly interested in this research and would have loved to see its conclusion.
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© 2011 Springer Science+Business Media, LLC
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Torrens, F., Castellano, G. (2011). Cooperativity of Protein Binding to Vesicles. In: Arabnia, H., Tran, QN. (eds) Software Tools and Algorithms for Biological Systems. Advances in Experimental Medicine and Biology, vol 696. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7046-6_27
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DOI: https://doi.org/10.1007/978-1-4419-7046-6_27
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