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Next-Day Salmonella spp. Detection Method Based on Real-Time PCR for Foods

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Salmonella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2182))

Abstract

Molecular techniques such as real-time polymerase chain reaction (qPCR) have become a very effective alternative in food microbiology diagnostic for rapid and specific detection of foodborne pathogens such as Salmonella in foods and food-related environments. qPCR is a simple, sensitive, specific, and reproducible assay. Here, we describe the application of real-time PCR-based methods for a rapid (less than 24 h) detection of Salmonella in different types of foods fully compatible with the international standard for detection of Salmonella in food (ISO 6579-1:2017).

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References

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  4. International Organization for Standardization (2017) ISO 6579-1:2017: Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp. Geneve

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Correspondence to David Rodríguez-Lázaro .

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Rodríguez-Lázaro, D., Hernández, M. (2021). Next-Day Salmonella spp. Detection Method Based on Real-Time PCR for Foods. In: Schatten, H. (eds) Salmonella. Methods in Molecular Biology, vol 2182. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0791-6_1

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  • DOI: https://doi.org/10.1007/978-1-0716-0791-6_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0790-9

  • Online ISBN: 978-1-0716-0791-6

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