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Approaches of T Cell Activation and Differentiation for CAR-T Cell Therapies

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Chimeric Antigen Receptor T Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2086))

Abstract

Chimeric antigen receptor (CAR) T cell therapies are ex vivo manufactured cellular products that have been useful in the treatment of blood cancers and solid tumors. The quality of the final cellular product is influenced by several amenable factors during the manufacturing process. This review discusses several of the influences on cell product phenotype, including the raw starting material, methods of activation and transduction, and culture supplementation.

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Authors and Affiliations

  1. Center for Cellular Immunotherapies, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Robert D. Schwab, Darel Martínez Bedoya, Tiffany R. King, Bruce L. Levine & Avery D. Posey Jr.

  2. Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Bruce L. Levine

  3. Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Avery D. Posey Jr.

  4. Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA

    Avery D. Posey Jr.

Authors
  1. Robert D. Schwab
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  2. Darel Martínez Bedoya
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  3. Tiffany R. King
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  4. Bruce L. Levine
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  5. Avery D. Posey Jr.
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Corresponding author

Correspondence to Avery D. Posey Jr. .

Editor information

Editors and Affiliations

  1. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

    Kamilla Swiech

  2. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

    Kelen Cristina Ribeiro Malmegrim

  3. Center for Cell-Based Therapy CTC Regional Blood Center of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

    Virgínia Picanço-Castro

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Schwab, R.D., Bedoya, D.M., King, T.R., Levine, B.L., Posey, A.D. (2020). Approaches of T Cell Activation and Differentiation for CAR-T Cell Therapies. In: Swiech, K., Malmegrim, K., Picanço-Castro, V. (eds) Chimeric Antigen Receptor T Cells. Methods in Molecular Biology, vol 2086. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0146-4_15

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  • DOI: https://doi.org/10.1007/978-1-0716-0146-4_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0145-7

  • Online ISBN: 978-1-0716-0146-4

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