Abstract
We have previously shown that green tea (GT) drinking combined with vitamin E supplementation reduced plasma protein carbonyls and increased erythrocytes catalase activity in exercising healthy elderly. In the present study we set out to investigate the antioxidative effects of GT drinking in an aging population. We performed an interventional, crossover, controlled prospective trial with 35 healthy elderly subjects (mean age 67.3 ± 4.8 years), supplemented with four daily placebo maltodextrin “tea-bags” for 12 weeks, followed by four 1.5 g daily GT bags for another 12 weeks. Data were obtained at baseline, at the end of the placebo period, and at the end of the GT intervention period. We found that GT did not alter erythrocyte catalase activity. However, it provided protection against 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis which declined by 10.2 % (p < 0.001). No changes were observed in saliva oral peroxidase enzymes. Nonetheless, saliva total antioxidant capacity increased by 42.0 % (p < 0.01). Plasma oxidative products, such as protein carbonyls, lipid peroxides and thiobarbituric acid reactive substances (TBARS) were stable throughout the intervention period. We conclude that four daily cups of GT are well tolerated in elderly free living subjects. Our results demonstrate that both erythrocyte resistances to oxidation and saliva antioxidant capacity are improved by GT drinking. The clinical implications of these oxidation modifications require further research.
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Acknowledgements
We thank Kibbutz Beit HaShita medical staff, Yoke Roded, Smadar Lustgarten and Hannah Shulami, for excellent assistance. This study was supported by the Krol foundation of Barnegat NJ, USA, Research and Scholarships Fund in Food and Nutrition Fields with Public Health Implication. Rappaport Institute for Research and Myers-JDC-Brookdale Institute of Gerontology and Human Development and Eshel-the Association for the Planning and Development of Services for the Aged in Israel and by Wissotzky Tea Company-Tel Aviv, Israel.
Conflicts of Interest Statement
Wissotzky Tea Company supported the study and provided placebo and GT bags. The company was not involved in any phase of the study including design and data analysis. The authors declare no other potential conflicts of interest in relation to this article.
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Narotzki, B., Reznick, A.Z., Mitki, T., Aizenbud, D., Levy, Y. (2014). Green Tea Drinking Improves Erythrocytes and Saliva Oxidative Status in the Elderly. In: Pokorski, M. (eds) Oxidative Stress and Cardiorespiratory Function. Advances in Experimental Medicine and Biology(), vol 832. Springer, Cham. https://doi.org/10.1007/5584_2014_8
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DOI: https://doi.org/10.1007/5584_2014_8
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