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DNA—lipid systems. An amphiphile self-assembly and polymer-surfactant perspective

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Lipid and Polymer-Lipid Systems

Part of the book series: Progress in Colloid and Polymer Science ((PROGCOLLOID,volume 120))

Abstract

The interaction between DNA and oppositely charged surfactants has been investigated by several techniques, like fluorescence microscopy, electron microscopy, phase diagram determination, and ellipsometry. The phase behaviour is more strongly associative than that in previously studied systems. A precipitate is formed for very low amounts of surfactant and DNA. DNA compaction is a general phenomenon in the presence of multivalent ions and positively charged surfaces; because of the high charge density there are strong attractive ion correlation effects. The interaction between DNA and catanionic mixtures (i.e., mixtures of cationic and anionic surfactants) was also investigated. We observed that DNA compacts and adsorbs onto the surface of positively charged vesicles and that the addition of anionic surfactant can release free DNA back into solution from a compact globular complex between DNA and cationic surfactant. Finally, we investigated DNA interactions with polycations, chitosans with different chain lengths, by fluorescence microscopy, in vivo transfections assays and cryogenic transmission electron microscopy. The general conclusion is that a chitosan effective in promoting compaction is also efficient in transfection.

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Author notes

  1. S. Mel’nikov

    Present address: Product Microstructure, Unilever Research Laboratorium, P.O. Box 114, 3130 AC, Vlaardingen, The Netherlands

  2. K. Eskilsson

    Present address: Institute of Surface Chemistry, P.O. Box 5607, 114 86, Stockholm, Sweden

Authors and Affiliations

  1. Physical Chemistry 1, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, 221 00, Lund, Sweden

    Bjorn Lindman, S. Mel’nikov, Y. Mel’nikova, T. Nylander & K. Eskilsson

  2. Chemistry Department, University of Coimbra, 3004-535, Coimbra, Portugal

    M. Miguel, R. Dias & C. Leal

Authors
  1. Bjorn Lindman
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  2. S. Mel’nikov
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  3. Y. Mel’nikova
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  4. T. Nylander
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  5. K. Eskilsson
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  6. M. Miguel
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  7. R. Dias
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  8. C. Leal
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Editor information

Tommy Nylander Björn Lindman

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© 2002 Springer-Verlag

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Lindman, B. et al. (2002). DNA—lipid systems. An amphiphile self-assembly and polymer-surfactant perspective. In: Nylander, T., Lindman, B. (eds) Lipid and Polymer-Lipid Systems. Progress in Colloid and Polymer Science, vol 120. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-45291-5_8

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  • DOI: https://doi.org/10.1007/3-540-45291-5_8

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-43001-8

  • Online ISBN: 978-3-540-45291-1

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