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Cytocompatible Hydrogel Composed of Phospholipid Polymers for Regulation of Cell Functions

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Polymers in Nanomedicine

Part of the book series: Advances in Polymer Science ((POLYMER,volume 247))

Abstract

We propose a cell encapsulation matrix for use with a cytocompatible phospholipid polymer hydrogel system for control of cell functions in three-dimensional (3D) cell engineering and for construction of well-organized tissue in vivo. In cell engineering fields, it is important to produce cells with highly cell-specific functions. To realize this, we consider that new devices are needed for cell culture. So, we have designed soft biodevices using a spontaneously forming and cytocompatible polymer hydrogel system. A water-soluble phospholipid polymer bearing a phenylboronic acid unit, poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-vinylphenylboronic acid) (PMBV), was prepared. This polymer, in aqueous solution, spontaneously converted to a hydrogel by addition of poly(vinyl alcohol) (PVA) aqueous solution due to reversible bonding between the boronate groups in PMBV and the hydroxyl groups in PVA. The PMBV/PVA hydrogel was dissociated by an exchange reaction with low molecular weight diol compounds such as d-fructose, which have high binding affinity to the phenylboronic acid unit. Cells were encapsulated easily in the PMBV/PVA hydrogel, and the cells in the hydrogel kept their original morphology and slightly proliferated during the preservation period. After dissociation of the hydrogel, the cells could be recovered as a cell suspension and cultured under conventional cell culture conditions as usual. Embryonic stem cells could be encapsulated without any adverse effects from the polymer hydrogel, i.e., the cells maintained their undifferentiated character during preservation in the PMBV/PVA hydrogel. Cell preservation and activity in the hydrogel were also investigated using microfluidic chips. The results clearly indicated that the PMBV/PVA hydrogels provide a useful platform for 3D encapsulation of cell culture systems without any reduction of their bioactivity.

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Acknowledgments

The authors appreciate Prof. Madoka Takai, Dr. Ryosuke Matsuno, Dr. Yuuki Inoue and Prof. Takehiko Kitamori at The University of Tokyo for helpful discussions during preparation of the manuscript. One of the authors, Dr. Xu Yan, moved to Osaka Prefecture University, Osaka, Japan in April 2011.

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Authors and Affiliations

  1. Department of Materials Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan

    Kazuhiko Ishihara

  2. Department of Bioengineering, The University of Tokyo, Bunkyo-ku, Tokyo, Japan

    Kazuhiko Ishihara & Tomohiro Konno

  3. Center for NanoBio Integration, The University of Tokyo, Bunkyo-ku, Tokyo, Japan

    Kazuhiko Ishihara, Yan Xu & Tomohiro Konno

  4. Core Research of Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Chiyoda-ku, Tokyo, Japan

    Kazuhiko Ishihara & Tomohiro Konno

  5. Department of Applied Chemistry, School of Engineering, Bunkyo-ku, Tokyo, Japan

    Yan Xu

Authors
  1. Kazuhiko Ishihara
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  2. Yan Xu
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  3. Tomohiro Konno
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Correspondence to Kazuhiko Ishihara .

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Editors and Affiliations

  1. Dept. of Polymer Science and Engineering, Kyoto Institute of Technology, Goshokaido-cho, Matsugasaki, Kyoto, 606-8585, Japan

    Shigeru Kunugi

  2. Department of Biomedical Engineering, National Cerebral and Cardiovascular Cen, Fujisirodai 5-7-1, Osaka, 565-8565, Japan

    Tetsuji Yamaoka

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Ishihara, K., Xu, Y., Konno, T. (2011). Cytocompatible Hydrogel Composed of Phospholipid Polymers for Regulation of Cell Functions. In: Kunugi, S., Yamaoka, T. (eds) Polymers in Nanomedicine. Advances in Polymer Science, vol 247. Springer, Berlin, Heidelberg. https://doi.org/10.1007/12_2011_151

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