Conclusion
The results of our study have shown that REP-like repetitive DNA elements are present in the genome of Proteus strains and the rep-PCR technique is useful for generating DNA fingerprints of Proteus penneri strains. Beside evident differences in DNA band patterns ofthe strains there were bands specific for all of the Proteus penneri isolates. Analysis of these results showed a large degree ofgenetic heterogenity among these bacteria. Furthermore, a PCR fingerprint technique was used in this study in order to characterise clinical Proteus penneri strains and to determine the applicability of this technique for identification and comparison of Proteus strains from clinical and other biological materials, for diagnostic and epidemiological purposes.
Additionally, because phenotypic expression ofvirulence determinants of bacterial strains may be affected by storage and culture conditions, such genotyping techniques may be also used as an additional tool for differentiation of strains from patients with different clinical disorders.
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Kowalczyk, M., Sidorczyk, Z. (2002). Determination of Genetic Diversity of Proteus Penneri Strains Using Rep-PCR. In: Emoődy, L., Pál, T., Hacker, J., Blum-Oehler, G. (eds) Genes and Proteins Underlying Microbial Urinary Tract Virulence. Advances in Experimental Medicine and Biology, vol 485. Springer, Boston, MA. https://doi.org/10.1007/0-306-46840-9_42
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DOI: https://doi.org/10.1007/0-306-46840-9_42
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