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Validation of the 13C-bicarbonate tracer technique for estimation of CO2 production and energy expenditure in mink (Neovison vison)

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Proceedings of the Xth International Scientific Congress in fur animal production

Abstract

The objective was to validate the 13C-bicarbonate tracer technique (13CT) for estimation of the CO2 production (RCO2) and energy expenditure (EE) against ‘the golden standard’ indirect calorimetry (IC), in mink in the fed state. Six male mink were measured in a Latin-square design, simultaneously by means of 13CT and IC. A dose of NaH13CO3 was administrated orally, by subcutaneous or intra muscular injection. A zero breath sample was collected before the isotope administration. After dose administration breath samples were collected at frequent intervals for 6 h, and simultaneously the animals were measured by IC for 22 h. There was excellent agreement between results from 13CT and IC, and when using the measured factor for recovery of 13C in breath and measured RQ values, no significant differences between administration methods in estimates of RCO2 and EE were found. The 13CT was concluded to be a useful method for estimating EE, regardless of administration method, but accuracy is dependent on the estimates used for RF and RQ.

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Acknowledgements

The authors wish to express their sincere gratitude to Merethe Stubgaard and Ebba de Neergaard Harrison for skilful performance of chemical analyses, Abdalla Ali for operation of the respiration and IRIS units, Boye K. Pedersen and Kim Dinesen for professional handling of the mink, and Dr. Peter Junghans for assistance with the calculations.

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Authors and Affiliations

  1. Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 3, 1870, Frederiksberg C, Denmark

    C. Larsson & A.-H. Tauson

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  1. C. Larsson
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  2. A.-H. Tauson
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Correspondence to A.-H. Tauson .

Editor information

P. F. Larsen S. H. Møller T. Clausen A. S. Hammer T. M. Lássen V. H. Nielsen A. H. Tauson L. L. Jeppesen S. W. Hansen J. Elnif J. Malmkvist

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© 2012 Wageningen Academic Publishers The Netherlands

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Larsson, C., Tauson, AH. (2012). Validation of the 13C-bicarbonate tracer technique for estimation of CO2 production and energy expenditure in mink (Neovison vison). In: Larsen, P.F., et al. Proceedings of the Xth International Scientific Congress in fur animal production. Wageningen Academic Publishers, Wageningen. https://doi.org/10.3920/978-90-8686-760-8_6

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