Two-dimensional proteomics as a tool to evaluate nutritional effects in farmed fish
The use of proteomics in fish research is at a relatively early stage compared to terrestrial vertebrates. It has already been applied in the field of fish nutrition, enabling to point out metabolic changes occurring in response to dietary manipulations such as a variation in energy content, dietary nitrogen composition, lipid composition or the incorporation of plant protein sources in aquafeeds (Gomez-Requeni et al., 2011; Hamza et al., 2010; Kolditz et al., 2008; Martin et al., 2003; Sveinsdottir and Gudmundsdottir 2010). Of particular interest in the developing finfish aquaculture industry is to understand the nutritional requirements of the faster growing and more fragile larval and juvenile stages. However, the application of the proteomic approach is more challenging during the larval stage, due to the small size of the individuals and thereby the considerable amount of material needed for such an analysis, especially when the focus is a specific tissue.
KeywordsProtein Spot Comparative Proteome Analysis Gilthead Seabream Senegalese Sole Fish Nutrition
NR, M de V and TSS acknowledge financial support from Fundação para a Ciência e Tecnologia, Portugal, through grants SFRH/BDP/65578/2009, SFRH/BD/40698/2007 and SFRH/BD/41392/2007 respectively. The experiments presented here were partly funded by Fundaãâo para a Ciência e Tecnologia, through projects PDTC/MAR/105152/2008 (SPECIAL_K), PPCDT/MAR/61623/2004 (SAARGO), PTDC/MAR/71685/2006 (HYDRAA), prize CERATONIA given by Caixa Geral de Depósitos and University of Algarve, and Research Council of Norway through project 165203/S40.
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