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Proceedings of the IV Advanced Ceramics and Applications Conference pp 387–400Cite as

The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells

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Abstract

Nanomaterials are nowadays widely used in various fields of biomedicine. Before the application of biomaterials they have to be tested and fulfill some criteria. The first tests that should be performed after synthesis of some new nanomaterial with potential application in biomedical fields are biocompatibility tests. The aim of this study was to examine the influence of nanomaterial calcium phosphate/poly-(dl-lactide-co-glycolide) (CP/PLGA) on proliferation and adherence of HeLa cells in culture. For both proliferation and adherence examination, cells were treated with suspension of nanomaterial CP/PLGA at concentrations 5, 50 and 500 µg/mL, made in cell culture medium. There were four different types of treatment: (1) cells incubated with CP/PLGA for 3 days; (2) cells incubated with CP/PLGA for 6 days; (3) cells incubated first with CP/PLGA for 3 days and then for the next 3 days with medium and (4) cells incubated first with medium for 3 days and then for the next 3 days with CP/PLGA. Three days after incubation of HeLa cells with different concentrations of CP/PLGA nanoparticles’ suspension, the concentration of 5 µg/mL had mild inhibitory effect on proliferation. Increasing CP/PLGA concentration, there was stimulatory effect on cells’ proliferation. With prolonged incubation period, this dose dependence is lost. The highest adherence of HeLa cells was observed when cells were incubated with the highest examined concentration of CP/PLGA suspension, in both 3-day and 6-day incubation period. Based on the results obtained in our study, we can conclude that the effect of the suspension of nanomaterial CP/PLGA on proliferation and adherence of HeLa cells depends on the duration of incubation with the cells as well as the material concentration and type of the treatment.

Keywords

  • Nanomaterial
  • CP/PLGA
  • Hela cells
  • Proliferation
  • Adherence

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Acknowledgments

This study was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Project Grant No. III 41017).

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Authors and Affiliations

  1. Faculty of Medicine, University of Niš, Blvd. Dr Zoran Đinđić 81, 18000, Niš, Serbia

    Jelena G. Najdanović, Stevo J. Najman, Sanja Stojanović & Jelena M. Živković

  2. Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Knez Mihailova 35/4, 11000, Belgrade, Serbia

    Nenad Ignjatović & Dragan Uskoković

  3. Faculty of Mechanical Engineering, University of Niš, Aleksandra Medvedeva 14, 18000, Niš, Serbia

    Miroslav Trajanović

Authors
  1. Jelena G. Najdanović
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  2. Stevo J. Najman
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  3. Sanja Stojanović
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  4. Jelena M. Živković
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  5. Nenad Ignjatović
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  6. Dragan Uskoković
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  7. Miroslav Trajanović
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Corresponding author

Correspondence to Stevo J. Najman .

Editor information

Editors and Affiliations

  1. Department of Materials, Faculty of Engg, Imperial College London Department of Materials, Faculty of Engg, London, United Kingdom

    Bill Lee

  2. University of Stuttgart , Stuttgart, Germany

    Rainer Gadow

  3. Faculty of Electronic Engg, University of Nis Faculty of Electronic Engg, Niš, Serbia

    Vojislav Mitic

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Najdanović, J.G. et al. (2017). The Influence of Nanomaterial Calcium Phosphate/poly-(dl-lactide-co-glycolide) on Proliferation and Adherence of HeLa Cells. In: Lee, B., Gadow, R., Mitic, V. (eds) Proceedings of the IV Advanced Ceramics and Applications Conference. Atlantis Press, Paris. https://doi.org/10.2991/978-94-6239-213-7_27

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  • DOI: https://doi.org/10.2991/978-94-6239-213-7_27

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