Abstract
The aim of this study was to analyze changes in the surface and chemical composition of Bio-Oss® granules after incubation in cell culture medium. Appropriate amount of Bio-Oss® material was incubated in Dulbecco’s Modified Eagle’s Medium (DMEM) at 37 °C for 3 days. After that, granules were dried, coated with gold and analyzed using scanning electron microscope (SEM) equipped with EDX (JEOL, JSM 5300). SEM analysis revealed that the surface of Bio-Oss® particles looks less grainy after incubation in DMEM. The impression is that an incubation in DMEM made erosion of cam bumps or that larger and wider reefs compared to the material before incubation are made by depositing of new material. Semi-quantitative analysis of calcium and phosphorous content in Bio-Oss® before and after incubation in DMEM was determined by EDX spectroscopy and results show that concentration of calcium and phosphorous ions increases after incubation in DMEM.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
M. Wiedmann-Al-Ahmad, R. Gutwald, N.C. Gellrich, U. Hübner, R. Schmelzeisen, Search for ideal biomaterials to cultivate human osteoblast-like cells for reconstructive surgery. J. Mater. Sci.: Mater. Med. 16, 57–66 (2005)
O. Trubiani, S. Fulle, T. Traini, M. Paludi, R. La Rovere, M. Orciani, S. Caputi, A. Piattelli, Functional assay, expression of growth factors and proteins modulating bone-arrangement in human osteoblasts seeded on an anorganic bovine bone biomaterial. Eur. Cells Mater. 20, 72–83 (2010)
G. Orsini, T. Traini, A. Scarano, M. Degidi, V. Perrotti, M. Piccirilli, Piattelli A Maxillary sinus augmentation with Bio-Oss® particles: a light, scanning, and transmission electron microscopy study in man. J. Biomed. Mater. Res. 74B, 448–457 (2005)
J. Bassil, N. Naaman, R. Lattouf, C. Kassis, S. Changotade, B. Baroukh, K. Senni, G. Godeau, Clinical, histological, and histomorphometrical analysis of maxillary sinus augmentation using inorganic bovine in humans: preliminary results. J. Oral Implantol. 39(1), 73–80 (2013)
K.A. Schlegel, G. Fichtner, S. Schultze-Mosgau, J. Wiltfang, Histologic findings in sinus augmentation with autogenous bone chips versus a bovine bone substitute. Int. J. Oral Maxillofac. Implants 18(1), 53–58 (2003)
T. Accorsi-Mendonça, M. Baltazar Conz, T. Cristina Barros, L.Á. de Sena, G. de Almeida Soares, J.M. Granjeiro, Physicochemical characterization of two deproteinized bovine xenografts. Braz. Oral Res. 22(1), 5–10 (2008)
Ž. Mitić, S. Najman, M. Cakić, Z. Ajduković, N. Ignjatović, R. Nikolić, G. Nikolić, S. Stojanović, M. Vukelić, M. Trajanović, J. Mol. Struct. 1074, 315–320 (2014)
Acknowledgement
This work was supported by the Ministry of Education, Science and Technological development of the Republic of Serbia (Grant III41017).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Atlantis Press and the author(s)
About this paper
Cite this paper
Stojanović, S., Mitić, Ž., Miljković, M., Rajković, J., Trajanović, M., Najman, S. (2016). SEM-EDX Analysis of Bio-Oss® Granules After Incubation In Cell Culture Medium. In: Lee, W., Gadow, R., Mitic, V., Obradovic, N. (eds) Proceedings of the III Advanced Ceramics and Applications Conference. Atlantis Press, Paris. https://doi.org/10.2991/978-94-6239-157-4_18
Download citation
DOI: https://doi.org/10.2991/978-94-6239-157-4_18
Published:
Publisher Name: Atlantis Press, Paris
Print ISBN: 978-94-6239-156-7
Online ISBN: 978-94-6239-157-4
eBook Packages: EngineeringEngineering (R0)