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Microarray Analysis of Novel Adrenergic Receptor Functions

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The Adrenergic Receptors

Part of the book series: The Receptors ((REC))

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Abstract

The advent of DNA microarray technology has provided a means to identify changes in the expression of thousands of genes simultaneously. This research tool has enabled investigators to study the effects of adrenergic receptor (AR) stimulation on gene expression on a large scale and has led to the identification of many genes that are regulated by adrenergic receptors (ARs). Microarrays have been used to compare the effects of α1A-AR, α1B-AR, and α1D-AR stimulation on gene expression. This work demonstrated that all three α1-AR subtypes commonly regulate many types of genes. However, genes that are regulated by only one or two α1-AR subtypes have also been identified. These data provide evidence that the physiological roles of the three α1-AR subtypes are not redundant despite their activation by the same ligand, use of common signal transduction pathways, and overlapping tissue distributions. Microarray studies have also identified genes that underlie AR-mediated regulation of the cell cycle, apoptosis, neuronal differentiation, cell hypertrophy, and other biological processes that are regulated by ARs. In addition, microarrays have identified changes in gene expression that accompany AR-mediated disease states, including cardiac hypertrophy, neurodegeneration, and hypermetabolism. The purpose of this chapter is to review how microarrays have contributed to our understanding of AR function at the genomic level.

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Author information

Authors and Affiliations

  1. Department of Pharmacology, College of Pharmacy, Ohio Northern University, Ada, OH

    Boyd Rorabaugh

  2. The Department of Physiology and Pharmacology, Northeastern Ohio Universities College of Medicine, Rootstown, OH

    June Yun

  3. Department of Molecular Cardiology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, OH

    Dianne M. Perez

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  1. Boyd Rorabaugh
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  2. June Yun
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  3. Dianne M. Perez
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Editors and Affiliations

  1. Department of Molecular Cardiology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, OH

    Dianne M. Perez

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© 2006 Humana Press Inc., Totowa, NJ

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Rorabaugh, B., Yun, J., Perez, D.M. (2006). Microarray Analysis of Novel Adrenergic Receptor Functions. In: Perez, D.M. (eds) The Adrenergic Receptors. The Receptors. Humana Press. https://doi.org/10.1385/1-59259-931-1:365

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