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Cardiovascular Genomics pp 247–268Cite as

Application of Viral Gene Transfer in Studies of Neurogenic Hypertension

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Part of the book series: Contemporary Cardiology ((CONCARD))

Abstract

This chapter reviews the use of viral gene transfer to disentangle the complexities of neurogenic hypertension. Viral gene manipulation allows lasting and controllable genetic manipulation in selected areas of the brain in different species and strains, including experiments in the spontaneously hypertensive rat, an established model of hypertension. Recent evidence indicates that, in contrast to pharmacological tools that may act on any cellular target within a given area of the brain, viral vectors deliver transgene in a nonuniform manner, and its concentration in different types of cells may vary greatly. This occurs as a result of both transductional tropism of a viral vector system and the transciptional activity of the promoter in different cellular types which are present in any brain nucleus. Properties of adenoviral and lentiviral vectors are compared and contrasted. Application of viral vectors for overexpression of biologically active molecules, expression of dominant negative proteins, pathway tracking, and other experiments to study central mechanisms of cardiovascular control are discussed. In summary, virally mediated gene delivery to the brain is a powerful research tool that can be used to address a wide range of questions related to mechanisms of human essential hypertension.

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Authors and Affiliations

  1. Department of Physiology, School of Medical Sciences, University of Bristol, Bristol, UK

    Sergey Kasparov MD, PhD & Julian F. R. Paton PhD

  2. Department of Pharmacology, School of Medical Sciences, Univesity of Bristol, Bristol, UK

    A. G. Teschemacher PhD

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  1. Sergey Kasparov MD, PhD
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  2. A. G. Teschemacher PhD
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  3. Julian F. R. Paton PhD
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Editors and Affiliations

  1. Department of Physiology and Functional Genomics, University of Florida College of Medicine, Gainesville, FL

    Mohan K. Rai ADA PhD

  2. Department of Physiology, School of Medical Sciences, University of Bristol, Bristol, UK

    Julian F. R. Paton PhD  & Sergey Kasparov MD, PhD  & 

  3. Department of Pharmacodynamics, University of Florida College of Pharmacy, Gainesville, FL

    Michael J. Katovich PhD

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© 2005 Humana Press Inc.

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Kasparov, S., Teschemacher, A.G., Paton, J.F.R. (2005). Application of Viral Gene Transfer in Studies of Neurogenic Hypertension. In: Rai, M.K., Paton, J.F.R., Kasparov, S., Katovich, M.J. (eds) Cardiovascular Genomics. Contemporary Cardiology. Humana Press. https://doi.org/10.1385/1-59259-883-8:247

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  • DOI: https://doi.org/10.1385/1-59259-883-8:247

  • Publisher Name: Humana Press

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