Metabolic flux analysis: A key methodology for systems biology of metabolism
Genome-wide analyses of mRNA, protein, or metabolite complements of biological systems produce unprecedented data sets. In contrast to such cellular composition data, in vivo quantification of molecular fluxes through metabolic networks links genes and proteins to higher-level functions that result from biochemical and regulatory interactions between network components. By unraveling novel or unexpected pathways in microbes, metabolic flux analyses begin to question the ability of well-known ’textbook’ pathways to portray flux through complex networks. Accumulating data on flux responses to genetic or environmental changes reveal general design principles and system properties of metabolic network operation. Beyond such discoveries, flux data assume increasingly important roles in completing network models and verifying or refuting their predictions. With recent advances in analytical accuracy, mathematical frameworks, available software, and experimental throughput, steady state flux analysis became a key methodology for metabolic systems biology.
KeywordsMetabolic Network Metabolic Flux Flux Analysis Corynebacterium Glutamicum Metabolic Flux Analysis
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