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Fine-Tuning of RNA Functions by Modification and Editing pp 179–204Cite as

Role of the 5’-cap in the biogenesis of spliceosomal snRNPs

Part of the Topics in Current Genetics book series (TCG,volume 12)

Abstract

The biogenesis of spliceosomal UsnRNPs in higher eukaryotes involves a nucleocytoplasmic shuttling cycle. After transcription and processing in the nucleus, the m7G-cap-dependent export of the snRNAs U1, U2, U4, and U5 to the cytoplasm occurs. In the cytoplasm, these UsnRNAs specifically associate with seven Sm-proteins and form a doughnut-shaped snRNP core structure. This assembly, mediated by the SMN complex, is a prerequisite for the hypermethylation of the m7G-cap to the 2,2,7-trimethylguanosine (m3G)-cap. Snurportin1 (SPN1), specifically, recognises the m3G-cap and facilitates the nuclear import of UsnRNPs. The recently determined crystal structure of human SPN1 reveals a significantly different binding mode for the cap structure in comparison to that of the m7G-binding proteins CBC, eIF4E and VP39.

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    Achim Dickmanns & Ralf Ficner

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Dickmanns, A., Ficner, R. Role of the 5’-cap in the biogenesis of spliceosomal snRNPs. In: Grosjean, H. (eds) Fine-Tuning of RNA Functions by Modification and Editing. Topics in Current Genetics, vol 12. Springer, Berlin, Heidelberg. https://doi.org/10.1007/b106799

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