Effect of surface charges on the electroporation process in lipid bilayers

  • M. Gambaro
  • A. Gliozzi
  • M. Robello
Part of the Progress in Colloid & Polymer Science book series (PROGCOLLOID, volume 84)


This paper presents a critical phenomenon involving a sharp increase in conductance at a critical value of the electric field applied to a bilayer lipid membrane (BLM). By varying the density and the sign of the charge on the BLM at various pH values, the critical potential changes. These changes are related to the variations in the surface potential and indicate that the reorganization of the external polar surface under the action of the electric field always starts on the side in which the electric field is directed from the inner to the outer surface of the monolayer.

Key words

Electroporation conductance transition titration of phospholipids black lipid membranes 


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  1. 1.
    Sowers AE (1989) In: Neumann E, Sowers AE, Jordan CA (eds) Electroporation and electrofusion in cell biology. Plenum Publishing corporation, pp 229–256Google Scholar
  2. 2.
    Glaser RW, Leikin SL, Chernomodik LV, Pastushenko VF, Sokirko AI (1988) Biochim et Biophys Acta 940:275–287CrossRefGoogle Scholar
  3. 3.
    Chernomodik LV, Chizmadzhev YuA (1989) In: [1] pp 83–95Google Scholar
  4. 4.
    Robello M, Gliozzi A (1989) Biochim et Biophys Acta 982:173–176CrossRefGoogle Scholar
  5. 5.
    Montal M, Mueller P, Proc Natl Acad Sci USA 69:3561–3566Google Scholar
  6. 6.
    Gabrielli G, Gliozzi A, Sanguineti A, D'Agata A (1989) Colloids and Surfaces 35:261–273CrossRefGoogle Scholar
  7. 7.
    Mirghani Z, Bertoia D, Gliozzi A, De Rosa M, Gambacorta A (1990) Chemistry and Physics of Lipids 55:85–96CrossRefGoogle Scholar
  8. 8.
    Fernandez MS, Celis H, Montal M (1973) Biochim et Bophys Acta 323:600–605CrossRefGoogle Scholar
  9. 9.
    Fernandez MS (1988) Biochim et Biophys Acta 942:199–204CrossRefGoogle Scholar

Copyright information

© Dr. Dietrich Steinkopff Verlag GmbH & Co. KG 1991

Authors and Affiliations

  • M. Gambaro
    • 1
  • A. Gliozzi
    • 1
  • M. Robello
    • 1
  1. 1.Dipartimento di FisicaUniversità di GenovaGenovaItaly

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