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Ultracentrifugation pp 40–45Cite as

Analysis of interaction of the small heat shock protein hsp25 with actin by analytical ultracentrifugation

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Part of the book series: Progress in Colloid & Polymer Science ((PROGCOLLOID,volume 94))

Abstract

By using analytical ultracentrifugation interactions of the small heat shock protein hsp25 and G-actin have been analyzed. Association constants of about 107 reciprocal molar concentration were estimated from the concentration distribution using a computer program based on the nonlinear least-squares methode. In the presence of an excess of actin up to four molecules of this protein were bound to hsp25 particles.

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Authors and Affiliations

  1. Max Delbrück Centrum für Molekulare Medizin, Robert-Rössle-Straße 10, 13121, Berlin

    J. Behlke, O. Ristau & A. Knespel

Authors
  1. J. Behlke
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  2. O. Ristau
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  3. A. Knespel
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Editor information

M. D. Lechner

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© 1994 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG

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Behlke, J., Ristau, O., Knespel, A. (1994). Analysis of interaction of the small heat shock protein hsp25 with actin by analytical ultracentrifugation. In: Lechner, M.D. (eds) Ultracentrifugation. Progress in Colloid & Polymer Science, vol 94. Steinkopff. https://doi.org/10.1007/BFb0115600

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  • DOI: https://doi.org/10.1007/BFb0115600

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  • Publisher Name: Steinkopff

  • Print ISBN: 978-3-7985-0982-5

  • Online ISBN: 978-3-7985-1675-5

  • eBook Packages: Springer Book Archive

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