Abstract
The inhibition effect of AOT reverse micelles in the p-nitrophenol ester hydrolysis, has been compared with lipases from Pseudomonas sp., and isoespecies A and B from Candida rugosa. These anionic micelles induced a displacement of the optimal pH of lipase in opposite direction to that observed with other hydrophilic non surface-active enzymes. A relation between the hydrophobic character of each lipase and a blue displacement of their fluorescence emission in micelles was found. Also, a decay of the emission fluorescence intensity was detected under experimental conditions at which lipases showed maximal activity. Lipases located in a more hydrophobic environment were less inhibited by the AOT system.
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Abbreviations
- AOT:
-
Sodium bis-(2-ethylhexyl) sulfosuccinate
- pNPB:
-
p-nitro-phenyl butyrate
- ω0 :
-
[H2O]/[surfactant]
- k 02 :
-
kcat/Km, app
- CCL:
-
Candida cylindracea or rugose lipase
- PSL:
-
Pseudomonas sp. lipase (lipase PS)
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© 1995 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG
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Otero, C., Robledo, L. (1995). Lipase activity in anionic reverse micelles. Inhibition effect of the system. In: Appell, J., Porte, G. (eds) Trends in Colloid and Interface Science IX. Progress in Colloid & Polymer Science, vol 98. Steinkopff. https://doi.org/10.1007/BFb0115242
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DOI: https://doi.org/10.1007/BFb0115242
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