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Lipase activity in anionic reverse micelles. Inhibition effect of the system

  • Biocolloids
  • Conference paper
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Book cover Trends in Colloid and Interface Science IX

Part of the book series: Progress in Colloid & Polymer Science ((PROGCOLLOID,volume 98))

Abstract

The inhibition effect of AOT reverse micelles in the p-nitrophenol ester hydrolysis, has been compared with lipases from Pseudomonas sp., and isoespecies A and B from Candida rugosa. These anionic micelles induced a displacement of the optimal pH of lipase in opposite direction to that observed with other hydrophilic non surface-active enzymes. A relation between the hydrophobic character of each lipase and a blue displacement of their fluorescence emission in micelles was found. Also, a decay of the emission fluorescence intensity was detected under experimental conditions at which lipases showed maximal activity. Lipases located in a more hydrophobic environment were less inhibited by the AOT system.

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Abbreviations

AOT:

Sodium bis-(2-ethylhexyl) sulfosuccinate

pNPB:

p-nitro-phenyl butyrate

ω0 :

[H2O]/[surfactant]

k 02 :

kcat/Km, app

CCL:

Candida cylindracea or rugose lipase

PSL:

Pseudomonas sp. lipase (lipase PS)

References

  1. Bradly L, Brzozowski AM, Derewenda ZS, Dodson E, Dodson G, Tolley S, Turkenburg JP, Christiansen L, Huge-Hensen B, Norskov L, Thim L, Menge U (1990) Nature 343:767–770

    Article  Google Scholar 

  2. Derewenda U, Brzozowski AM, Lawson DM, Derewenda ZS (1992) Biochemistry 31:1532–1541

    Article  CAS  Google Scholar 

  3. Rúa ML, Díaz-Mauriño T, Otero C, Ballesteros A (1992) Ann NY Acad Sci 672:20–23

    Article  Google Scholar 

  4. Rúa ML, Díaz-Mauriño T, Fernández VM, Otero C, Ballesteros A (1993) Biochem Biophys Acta 1156:181–189

    Google Scholar 

  5. Otero C, Rua ML, Robledo L (1995) FEBS Letters 360:202–206

    Article  CAS  Google Scholar 

  6. Longhi S, Fusetti F, Grandori R, Lotti M, Vanoni M, Alberghina L (1992) Biochim Biophys Acta 1131:227–232

    CAS  Google Scholar 

  7. Lotti M, Tramontano A, Longhi S, Fussetti T, Brocca S, Pizzi E, Alberghina L (1994) Protein Engineering 7(4):531–535

    Article  CAS  Google Scholar 

  8. Khmelnitsky YuL, Kabanov AV, Klyachko NL, Levashov AV, Martinek K, in Pileni MP, Troyanowsky C (eds) Structure and Reactivity in Reverse Micelles Elsevier. Amsterdam

    Google Scholar 

  9. Fletcher PDI, Robinson BH, Freedman RB, Oldfield C (1985) J Chem Soc Faraday Trans 81:2667–2679

    Article  CAS  Google Scholar 

  10. Oldfield C (1987) Ph.D. University of Kent (UK)

    Google Scholar 

  11. Castro MJM, Cabral JMS; Enzyme Microb Technol 11:6–11

    Google Scholar 

  12. Kuntz ID, Kauzmann W (1974) Adv Protein Chem 28:239–345

    Article  CAS  Google Scholar 

  13. Bru R, Sanchez-Ferrer A, García-Carmona F (1989) Biotech Bioeng 34:304–308

    Article  CAS  Google Scholar 

  14. Ohshima A, Narita H, Kito M (1983) J Biochem 93:1421–1425

    CAS  Google Scholar 

  15. Bru R, Sanchez-Ferrer A, García-Carmona F (1989) Biochem J 259: 355–361

    CAS  Google Scholar 

  16. Schmidli PK, Luisi PL (1990) Biocatalysis 3:367–376

    Article  CAS  Google Scholar 

  17. Oldfield C, Freedman RB (1989) Eur J Biochem 183:347–355

    Article  CAS  Google Scholar 

  18. Grochulski P, Li Y, Schrag JD, Bouthillier F, Smith P, Harrison D, Rubin B, Cygler M (1993) J Biol Chem 268:12843–12847

    CAS  Google Scholar 

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Authors and Affiliations

  1. Instituto de Catálisis CSIC, Campus de la Universidad Autonoma, 28049, Cantoblanco, Spain

    C. Otero & L. Robledo

Authors
  1. C. Otero
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  2. L. Robledo
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Editor information

J. Appell G. Porte

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© 1995 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG

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Otero, C., Robledo, L. (1995). Lipase activity in anionic reverse micelles. Inhibition effect of the system. In: Appell, J., Porte, G. (eds) Trends in Colloid and Interface Science IX. Progress in Colloid & Polymer Science, vol 98. Steinkopff. https://doi.org/10.1007/BFb0115242

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  • DOI: https://doi.org/10.1007/BFb0115242

  • Published:

  • Publisher Name: Steinkopff

  • Print ISBN: 978-3-7985-1031-9

  • Online ISBN: 978-3-7985-1667-0

  • eBook Packages: Springer Book Archive

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