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X-ray studies on biological membranes using synchrotron radiation

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Synchrotron Radiation in Chemistry and Biology I

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 145))

Abstract

Current views on the structure and function of biological membranes imply a great variety of dynamical aspects. These range from the dynamic phase behaviour of phospholipids and conformational variations of membrane proteins during membrane-associated processes, to the interactions between membranes in fusion and pore formation. The structural description of these processes, many of which imply the possibility of intermediate structures, calls for fast time-resolved diffraction methods, i.e. the cinematographic approach. The present article gives first an overview on the specific problems and the theories for their solution in X-ray diffraction on membranes, and then reviews the present possibilities of time-resolved structural studies using synchrotron radiation on phospholipid model systems and functional membranes (sarcoplasmic reticulum membrane). It is shown that such studies bear great promise in entering the millisecond time domain and bridging the existing gap between static structural information and the wealth of dynamic data derived from spectroscopic methods.

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  1. Institut für Röntgenfeinstrukturforschung der Österreichischen Akademie der Wissenschaften und des Forschungszentrums Graz Steyrergasse 17, A-8010, Graz, Austria

    Peter Laggner

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dedicated to Professor Otto Kratky on the occasion of his eightyfifth birthday.

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© 1988 Springer-Verlag

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Laggner, P. (1988). X-ray studies on biological membranes using synchrotron radiation. In: Synchrotron Radiation in Chemistry and Biology I. Topics in Current Chemistry, vol 145. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0111240

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  • DOI: https://doi.org/10.1007/BFb0111240

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  • Print ISBN: 978-3-540-18385-3

  • Online ISBN: 978-3-540-47935-2

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