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Pseudomonas cepacia lipase localization in lecitihin and AOT w/o microemulsions. A fluorescence energy transfer study

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Book cover Trends in Colloid and Interface Science XI

Part of the book series: Progress in Colloid & Polymer Science ((PROGCOLLOID,volume 105))

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Abstract

Two water-in-oil microemulsion systems formulated with lecithin and AOT were used to solubilize lipase from Pseudomonas cepacia. The site of localization of the enzyme within the different microdomains of the dispersed phase was investigated by applying the fluorescence energy transfer technique. The nonradiative energy transfer from the tryptophan residues of lipase to an adequate acceptor molecule, such as cis-parinaric acid, was studied in both systems. The effect of the water content of the microemulsions, as well as the effect of the molar ratio of lipase to cis-parinaric acid was examined. The results showed that in both microemulsion systems lipase is preferentially localized near the surfactant membrane and that in the case of the lecithin-based systems the interface is less penetrable than the AOT one.

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Authors and Affiliations

  1. Institute of Biological Research and Biotechnology National Hellenic, Research Foundation 48, Vas. Constantinou Ave, 11635, Athens, Greece

    S. Avramiotis, H. Stamatis & Dr. A. Xenakis

  2. Department of Chemical Engineering Biosystems Technology Lab. Zografou Campus, National Technical University of Athens, 11635, Athens, Greece

    H. Stamatis & F. N. Kolisis

Authors
  1. S. Avramiotis
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  2. H. Stamatis
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  3. Dr. A. Xenakis
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  4. F. N. Kolisis
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Editor information

J. B. Rosenholm B. Lindman P. Stenius

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© 1997 Steinkopff Verlag

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Avramiotis, S., Stamatis, H., Xenakis, A., Kolisis, F.N. (1997). Pseudomonas cepacia lipase localization in lecitihin and AOT w/o microemulsions. A fluorescence energy transfer study. In: Rosenholm, J.B., Lindman, B., Stenius, P. (eds) Trends in Colloid and Interface Science XI. Progress in Colloid & Polymer Science, vol 105. Steinkopff. https://doi.org/10.1007/BFb0110984

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  • DOI: https://doi.org/10.1007/BFb0110984

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  • Publisher Name: Steinkopff

  • Print ISBN: 978-3-7985-1086-9

  • Online ISBN: 978-3-7985-1660-1

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