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Fluidized bed adsorption as a primary recovery step in protein purification

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Book cover New Enzymes for Organic Synthesis

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((4143,volume 58))

Abstract

Fluidized bed adsorption has been introduced as an integrative technology combining clarification, concentration, and initial purification in a single step. In the paper presented here, the use of fluidized adsorbents in the primary recovery of proteins starting from unclarified broths is reviewed. First the principle of fluidizing adsorbent, particles is discussed, subsequently possible experimental procedures for whole broth adsorption are demonstrated. The system parameters governing the performance of the sorption process in a fluidized bed are discussed in the second part of the paper and considerations on how operating parameters and process design influence the limiting steps are provided. Finally, examples for the successful operation of whole broth adsorption, employing fluidized adsorbents are shown and conditions are defined under which this technology may be an alternative to traditional protein purification methods.

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Abbreviations

α:

proportionality constant in Eq. (19)

ε:

interstitial volume

η:

dynamic viscosity [kg/(m·s)]

ρl :

liquid density [kg/m3]

ρp :

solids density [kg/m3]

μ1 :

first central moment of the residence time distribution

μ2 :

second central moment of the residence time distribution

Bo:

Bodenstein number U·L/D axl

Ga:

Gallileo number ρgg(ρp-ρl)d 3p /η2

Pep :

Particle Peclet number U e ·d p /D axl

Rep :

Particle Reynolds number U·dp·ρl/η

Remf :

Reynolds number at minimum fluidization velocity

Ret :

Reynolds number at terminal setting velocity

Sc:

Schmidt number η/(D·ρl

BSA:

bovine serum albumin

CIP:

cleaning in place

D:

diffusion coefficient in free solution [m2/s]

Daxl :

liquid phase dispersion coefficient [m2/s]

Daxp :

solid phase dispersion coefficient [m2/s]

De :

effective (pore) diffusion coefficient [m2/s]

dc :

column diameter [m]

dp :

particle diameter [m]

G6PDH:

glucose-6-phosphate dehydrogenase

HSA:

human serum albumin

IP:

isoelectric point

K:

equilibrium constant

kf :

film transport coefficient [m/s]

L:

fluidized bed height [m]

m:

proportionality constant in Eq. (19)

MA :

protein molecular weight [kDa]

MAb:

monoclonal antibody

MDH:

malate dehydrogenase

MSFB:

magnetically stabilized fluidized bed

Mv:

density ratio (ρp-ρl)/ρl

n:

Richardson-Zaki coefficient

N L :

fluid side mass transport number

NP :

particle side mass transport number

Qdyn :

dynamic capacity [mg/mladsorbent]

Qmax :

equilibrium capacity [mg/mladsorbent]

rp :

particle radius [m]

SSRFB:

single stage recirculating fluidized bed

T:

absolute temperature [K]

U:

linear velocity [m/s]

Ue :

effective linear velocity (U/ε) [m/s]

Umf :

minimum fluidization velocity [m/s]

Ut :

terminal settling velocity [m/s]

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  1. Institut für Enzymetechnologie, Heinrich-Heine Universität Düsseldorf, D-52404, Jülich, Germany

    Jörg Thömmes

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Dedicated to Professor Dr. Maria-Regina Kula on the occasion of her 60th birthday

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Thömmes, J. (1997). Fluidized bed adsorption as a primary recovery step in protein purification. In: New Enzymes for Organic Synthesis. Advances in Biochemical Engineering/Biotechnology, vol 58. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0103305

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  • DOI: https://doi.org/10.1007/BFb0103305

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