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Vertebrate Cell Culture II and Enzyme Technology pp 73–95Cite as

Microbeads and anchorage-dependent eukaryotic cells: The beginning of a new era in biotechnology

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Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 39/1))

Abstract

Modern methods for the mass cultivation of anchorage-dependent mammalian cells started with the advent of microcarrier technology. Largely for reasons pertaining to their mode of preparation and ease of cultivation, 150–230 Μm microbeads have been overwhelmingly adopted and the technology around them developed. To meet high biomass, macroporous microbeads have been developed. Also, the chemistry of the microsupport has been adapted in order to afford better protection of fragile cells to mechanical wear while simultaneously reorienting their differentiation towards the sought aims (production of cytokines, enzymes etc. ...). Future progress depends upon solutions being brought to problems inherent to this new technology (maintenance of steady state conditions of growth etc. ...) as well as to requirements arising from animal cell culture in general (biosensors, bioreactor’s design etc. ...). Besides such technical implementations, biology at large is also expected to benefit from the advent of microcarriers in fields as diverse as the preparation of metaphasic chromosomes in bulk, toxicity testing, organ reconstitution following cell transplantation etc. h.

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  1. Laboratoire de Biochimie Moléculaire, Université de l’Etat 24, Avenue du Champ de Mars, 7000, Mons, Belgique

    A. O. A. Miller & F. D. Menozzi

  2. Unité de Biotechnologie Appliquée, Faculté de Médecine, Université de l’Etat 24, Avenue du Champ de Mars, 7000, Mons, Belgique

    D. Dubois

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Miller, A.O.A., Menozzi, F.D., Dubois, D. (1989). Microbeads and anchorage-dependent eukaryotic cells: The beginning of a new era in biotechnology. In: Vertebrate Cell Culture II and Enzyme Technology. Advances in Biochemical Engineering/Biotechnology, vol 39/1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0051952

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