Skip to main content

Analysis of coupled multi-image information in microscopy

  • Conference paper
  • First Online:
Book cover Visualization in Biomedical Computing (VBC 1996)

Part of the book series: Lecture Notes in Computer Science ((LNCS,volume 1131))

Included in the following conference series:

Abstract

Series of microscope recordings of cells, labeled for many different molecules, contain important biological information. The correct interpretation of those coupled multi-images, however, is not directly possible. This paper introduces a procedure for the analysis of higher-level combinatorical receptor patterns in the cellular immune system, which were obtained using the fluorescence multi-epitope-imaging microscopy. The cell recognition and the classification algorithm with an artificial neural network is described.

This work was supported by the DFG/BMBF grant (Innovationskolleg 15/A1).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Methods of Immunological Analysis, Cells and Tissues, Volume 3, Ed. by R.F. Masseyff; W.H. Albert; N.A. Staines, VCH-NewYork, 1993

    Google Scholar 

  2. W. Schubert: Antigenetic determinants of T lymphocyte αβ receptors and other leucocyte surface proteins as differential markers of skeletal muscle regeneration: detection of spatially and timely restricted patterns by MAM microscopy, Eur. J. Cell Biol. 62, pp. 395–410, 1993

    Google Scholar 

  3. W. Schubert, H. Schwan: Detection by 4-parameter microscopic imaging and increase of rare mononuclear blood leucocyte types expressing the γ receptor (CD16) for immunoglobulin G in human sporadic amyotrophic lateral sclerosis (ALS), Neurosci. Lett. 198, pp. 29–32, 1995

    Article  Google Scholar 

  4. J. Sklansky: On the Hough Technique for Curve Detection, IEEE Trans. on Comp. 27 (10), pp. 923–926, 1978

    MATH  Google Scholar 

  5. T. Kohonen: Self-Organizing Maps, Springer Series in Information Sciences, Springer, New York 1995

    Google Scholar 

  6. K. Obermayer, et.al.: Statistical-Mechanical Analysis of Self-Organization and Pattern Formation During the Development of Visual Maps, Physical Review A, Vol. 45(10), pp. 7568–7589, 1992

    Article  Google Scholar 

  7. Ch. Rethfeldt, et.al.: Multi-Dimensional Cluster Analysis of Higher-Level Differentiation at Cell-Surfaces, Proc. 1. Kongress der Neurowissenschaftlichen Gesellschaft, p.170, Spectrum Akademischer Verlag, Berlin 1996

    Google Scholar 

  8. St. Schünemann, B. Michaelis: A Self-Organizing Map for Analysis of High-Dimensional Feature Spaces with Clusters of Highly Differing Feature Density, Proc. 4th European Symposium on Artificial Neural Networks, pp. 79–84, Bruges 1996

    Google Scholar 

  9. St. Schünemann, et.al.: Analysis of Multi-Fluorescence Signals Using a Modified Self-Organizing Feature Map, accepted for: The Int. Conf. on Artificial Neural Networks, July 16–19, 1996, Bochum, Germany

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Karl Heinz Höhne Ron Kikinis

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

St. Schünemann, Rethfeldt, C., Müller, F., Agha-Amiri, K., Michaelis, B., Schubert, W. (1996). Analysis of coupled multi-image information in microscopy. In: Höhne, K.H., Kikinis, R. (eds) Visualization in Biomedical Computing. VBC 1996. Lecture Notes in Computer Science, vol 1131. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0046949

Download citation

  • DOI: https://doi.org/10.1007/BFb0046949

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-61649-8

  • Online ISBN: 978-3-540-70739-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics