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Novel separations based on affinity interactions

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Bioseparation

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 47))

Abstract

Novel purification processes have been developed, based on the affinity interaction between complementary molecules, to circumvent the difficulties associated with affinity chromatography. Depending upon the procedure used for isolating the ligand-ligate complex, the process can be termed affinity ultrafiltration, affinity partitioning or affinity precipitation. This review describes the developments, potentials, and applications of such purification procedures. Emphasis is also placed on the type and choice of affinity ligands as well as some common procedures by which ligands can be covalently immobilized to water-soluble or insoluble matrices. Finally, the problems and challenges encountered by such novel purification procedures are presented and discussed.

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Authors and Affiliations

  1. Biotechnology Research Institute National Research Council Canada, H4P 2R2, Montreal, Quebec, Canada

    J. H. T. Luong & A. -L. Nguyen

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  1. J. H. T. Luong
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  2. A. -L. Nguyen
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G. T. Tsao

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© 1992 Springer-Verlag

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Luong, J.H.T., Nguyen, A.L. (1992). Novel separations based on affinity interactions. In: Tsao, G.T. (eds) Bioseparation. Advances in Biochemical Engineering/Biotechnology, vol 47. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0046199

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  • DOI: https://doi.org/10.1007/BFb0046199

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-55551-3

  • Online ISBN: 978-3-540-47211-7

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