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Intracellular lytic enzyme systems and their use for disruption of Escherichia coli

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Applied Molecular Genetics

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 43))

Abstract

This article focusses on lytic enzyme systems available in E. coli and their potential use for cellular disruption. In the systems described here the genetic information for lysis would be carried within the microbial host, either integrated or naturally occurring on chromosomal DNA, or on extrachromosomal elements such as plasmids. Each microbe would carry complete information for endogenous enzymatic lysis, and lysis would occur in a controlled manner after being triggered by an external factor such as temperature or inducer addition. The lytic systems explored in this review include the autolytic enzymes, colicin lytic enzymes, and bacteriophage lytic enzymes from phage phiX174, T4, lambda, MS2 and Qβ. Many of the colicin lytic enzymes and all of the bacteriophage lytic enzymes described here have been cloned, and in some instances examined as cellular disruption methods. None of the E. coli autolytic enzymes have been cloned, but information pertinent for use as a disruption method is described.

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Authors and Affiliations

  1. Merck and Co., Inc., P.O. Box 7, 22827, Elkton, VA

    R. L. Dabora

  2. Department of Chemical Engineering and Biotechnology Process Engineering Center, Massachusetts Institute of Technology, 02139, Cambridge, MA

    C. L. Cooney

Authors
  1. R. L. Dabora
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  2. C. L. Cooney
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© 1990 Springer-Verlag

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Dabora, R.L., Cooney, C.L. (1990). Intracellular lytic enzyme systems and their use for disruption of Escherichia coli . In: Applied Molecular Genetics. Advances in Biochemical Engineering/Biotechnology, vol 43. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0009077

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  • DOI: https://doi.org/10.1007/BFb0009077

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  • Print ISBN: 978-3-540-52794-7

  • Online ISBN: 978-3-540-47151-6

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