Abstract
Soft tissue remodelling is the response of mechanical signal sensed by the molecular structure. However, the response of soft tissue molecular structure against the external mechanical stimulation is not well known. Some studies illustrated that the tissue remodelling depends on the rate of mechanical stimulation, however its cause is not well known. Understanding the response of molecular structure against the mechanical stimulation is crucial for the development of physiotherapeutic criteria to improve tissue health and wound healing. In this study, we investigated the effect of rate of mechanical stimulation on the alteration of nano and molecular structure of skin. For mechanical stimulation, pig skin was cyclically stretched at three different frequencies 0.1, 2.0 and 5.0 Hz. Whereas, nano and molecular structure of virgin and mechanical tested skin was assessed under atomic force microscope (AFM) and attenuated total reflectance Fourier transform infrared spectroscopy (FTIR–ATR), respectively. The alteration in nano and molecular structure was found sensitive to rate of mechanical stimulation as change in collagen fibril d-periodicity and collagen molecular (tropocollagen, protein structure) was found significantly larger corresponding to 0.1 Hz than 2.0 Hz and 5.0 Hz. A significant negative correlation (r = 0.74, p = 0.041) was found between stretching frequency and alteration in collagen protein structure. These results indicate that slow mechanical stimulation during physiotherapy may improve the tissue remodelling.
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References
Bonnans, C., Chou, J. and Werb, Z., 2014. Remodelling the extracellular matrix in development and disease. Nature reviews Molecular cell biology, 15(12), pp.786-801.
Bose, S., Li, S., Mele, E. and Silberschmidt, V.V., 2022. Exploring the mechanical properties and performance of Type-I collagen at various length scales: a progress report. Materials, 15(8), p.2753.
Buehler, M.J., 2006. Atomistic and continuum modeling of mechanical properties of collagen: elasticity, fracture, and self-assembly. Journal of Materials Research, 21(8), pp.1947-1961.
Dwivedi, K.K, Lakhani, P., Sihota, P., Tikoo, K., Kumar, S. and Kumar, N., 2023. The multiscale characterization and constitutive modeling of healthy and type 2 diabetes mellitus Sprague Dawley rat skin. Acta Biomaterialia, 158, pp.324-346.
Dwivedi, K.K., Lakhani, P., Kumar, S. and Kumar, N., 2020a. Frequency dependent inelastic response of collagen architecture of pig dermis under cyclic tensile loading: An experimental study. Journal of the Mechanical Behavior of Biomedical Materials, 112, p.104030
Dwivedi, K.K., Lakhani, P., Kumar, S. and Kumar, N., 2020b. The effect of strain rate on the stress relaxation of the pig dermis: a hyper-viscoelastic approach. Journal of Biomechanical Engineering, 142(9), p.091006.
Dwivedi, K.K., Lakhani, P., Kumar, S. and Kumar, N., 2022a. A hyperelastic model to capture the mechanical behaviour and histological aspects of the soft tissues. Journal of the Mechanical Behavior of Biomedical Materials, 126, p.105013.
Dwivedi, K.K., Lakhani, P., Kumar, S. and Kumar, N., 2022b. Effect of collagen fibre orientation on the Poisson's ratio and stress relaxation of skin: an ex vivo and in vivo study. Royal Society Open Science, 9(3), p.211301.
Fung, Y.C., 2013. Biomechanics: mechanical properties of living tissues. Springer Science & Business Media.
Gautieri, A., Vesentini, S., Redaelli, A. and Buehler, M.J., 2012. Viscoelastic properties of model segments of collagen molecules. Matrix Biology, 31(2), pp.141-149.
Hollingsworth, S.A. and Karplus, P.A., 2010. A fresh look at the Ramachandran plot and the occurrence of standard structures in proteins.
Jhorar, R. and Lamba, C.S., 2022. A Review on the Deformation Mechanism of Soft Tissue Collagen Molecules: An Atomistic Scale Experimental and Simulation Approaches. Forcefields for Atomistic-Scale Simulations: Materials and Applications, pp.269–297.
Kuehlmann, B., Bonham, C.A., Zucal, I., Prantl, L. and Gurtner, G.C., 2020. Mechanotransduction in wound healing and fibrosis. Journal of clinical medicine, 9(5), p.1423.
Lakhani, P., Dwivedi, K.K. and Kumar, N., 2020. Directional dependent variation in mechanical properties of planar anisotropic porcine skin tissue. Journal of the mechanical behavior of biomedical materials, 104, p.103693.
Lakhani, P., Dwivedi, K.K., Parashar, A. and Kumar, N., 2021. Non-invasive in vivo quantification of directional dependent variation in mechanical properties for human skin. Frontiers in bioengineering and biotechnology, 9, p.749492.
Rabotyagova, O.S., Cebe, P. and Kaplan, D.L., 2008. Collagen structural hierarchy and susceptibility to degradation by ultraviolet radiation. Materials Science and Engineering: C, 28(8), pp.1420-1429.
Sheng, R., Jiang, Y., Backman, L.J., Zhang, W. and Chen, J., 2020. The application of mechanical stimulations in tendon tissue engineering. Stem cells international, 2020.
Sherman, V.R., Yang, W. and Meyers, M.A., 2015. The materials science of collagen. Journal of the mechanical behavior of biomedical materials, 52, pp.22-50.
Uniyal, P., Sihota, P. and Kumar, N., 2022. Effect of organic matrix alteration on strain rate dependent mechanical behaviour of cortical bone. Journal of the Mechanical Behavior of Biomedical Materials, 125, p.104910.
Yang, W., Sherman, V.R., Gludovatz, B., Schaible, E., Stewart, P., Ritchie, R.O. and Meyers, M.A., 2015. On the tear resistance of skin. Nature communications, 6(1), p.6649.
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Dwivedi, K.K., Jhorar, R., Kumar, S., Kumar, N. (2024). Mechanical Stimulation Alters the Collagen Protein Structure in Skin Tissue. In: Gupta, J., Verma, A. (eds) Microbiology-2.0 Update for a Sustainable Future. Springer, Singapore. https://doi.org/10.1007/978-981-99-9617-9_20
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DOI: https://doi.org/10.1007/978-981-99-9617-9_20
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