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Application of CRISPR-Cas9 Technology in Fish

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Biotechnological Tools in Fisheries and Aquatic Health Management

Abstract

CRISPR/Cas9-based genome editing allows efficient and targeted enhancement of important aquaculture traits and will probably be the solution to the many present problems in the aquaculture industry. CRISPR/Cas9 has its own advantage over previously used genome editing platform like zinc finger nuclease (ZNF), meganuclease and transcription activator-like effector nuclease (TALEN) in terms of cost effectiveness, designing of construct, ease of delivery of construct and minimising the off-target modification. CRISPR/Cas9-based modification of growth-associated genes in important aquaculture species like red sea bream, channel catfish, common carp, etc. has shown promising result. Successful modifications were done also in grass carp, channel cat fish and zebra fish for acquiring disease resistance and stress tolerance. Apart from the growth- and disease-related traits, several studies were done to improve flesh quality, improve pigmentation, etc. in fish as well as in crustaceans. Besides that, knockout study with CRISPR/Cas9 system revealed function of many genes which were previously unknown. Highly efficient techniques for disease diagnosis are developed for detection of viral DNA/RNA in host cell based on CRISPR/Cas9-based system. In spite of wide-scale application of CRISPR/Cas9 system, there are a few challenges it may face in the genome modifications in fish which will be required to be resolved in order to achieve the full potential benefits of this wonderful tool of nature.

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Aich, N., Parhi, J., Mandal, S.C., Sahoo, L. (2023). Application of CRISPR-Cas9 Technology in Fish. In: Behera, B.K. (eds) Biotechnological Tools in Fisheries and Aquatic Health Management. Springer, Singapore. https://doi.org/10.1007/978-981-99-2981-8_2

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