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Research on Nonlinear Magnetization Detection Technology for Magnetic Immunoassay

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The Proceedings of the 17th Annual Conference of China Electrotechnical Society (ACCES 2022)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 1012))

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Abstract

A magnetic immunoassay system with simple structure, high accuracy and sensitivity, flexible signal frequency and amplitude was developed based on AC magnetic susceptibility and biotin-streptavidin system. During the immunohistochemical process of streptavidin superparamagnetic particles, biotinylated sheep anti-rabbit IgG and rabbit IgG, the characteristic frequency of magnetic complex changed as the hydrodynamic radius changed. Furthermore, the immunohistochemical process was reflected by relaxation time. This paper mainly carried out the immunohistochemical experiment of streptavidin super-paramagnetic particles. The experimental results show that the minimum detection limit of the system is 4.1000 mg/ml. When the viscosity coefficient, temperature and frequency of the solution are constant, AC susceptibility is positively correlated with the concentration. The characteristic frequency of the AC susceptibility’s imaginary part has no connection with the concentration. With the progress of immunohistochemistry, the characteristic frequency moved in the direction of decreasing frequency. The system provides a new idea for small molecule detection in application scenarios such as environmental monitoring and medical diagnosis.

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Correspondence to Ming Wang .

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Zhao, C., Wang, M., Xu, K., Feng, S. (2023). Research on Nonlinear Magnetization Detection Technology for Magnetic Immunoassay. In: Yang, Q., Li, J., Xie, K., Hu, J. (eds) The Proceedings of the 17th Annual Conference of China Electrotechnical Society. ACCES 2022. Lecture Notes in Electrical Engineering, vol 1012. Springer, Singapore. https://doi.org/10.1007/978-981-99-0357-3_8

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  • DOI: https://doi.org/10.1007/978-981-99-0357-3_8

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  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-99-0356-6

  • Online ISBN: 978-981-99-0357-3

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