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Principles of Basic and Advanced Techniques Used for the Purification of Enzymes of Industrial Importance

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Recent Advances in Bioprocess Engineering and Bioreactor Design

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Abstract

Recovery and purification are very critical and essential steps in the downstream processing (DSP) of industrially important enzymes and compounds. Purification process involves the enrichment of enzyme proportion by several hundred folds by employing various biophysical–biochemical tools and techniques. The purity and yield depend on the efficacy of suitable methods used in isolation, purification, and packaging of the end product. In the current topic, we have discussed the basic principles and workflow of enzymes isolation and their purification that involves various methods that includes cell disruption, centrifugation, filtration, and precipitation (by inorganic or organic solvents or isoelectric pH) in combination with various types of chromatographic techniques. It is very essential to verify the purity and activity of enzyme at various stages of purification steps by PAGE gel analysis and total activity and specific activity of enzymes. The downstream processing for some of the industrially important enzymes such as amylase, glucose isomerase, lipase, and pharmaceutically important enzymes have been discussed. Thus, understanding the basic principles of enzyme purification will definitely help in designing strategies that can influence the overall economy of enzyme manufacturing industry and eases the handling process.

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Authors and Affiliations

  1. Department of Biochemistry and Bioinformatics, GITAM School of Sciences, GITAM Deemed to be University, Visakhapatnam, India

    Kongara Hanumantha Rao

  2. Department of Biotechnology, School of Bioscience and Bioengineering, Lovely Professional University, Jhalandar, India

    S. Haseena

Authors
  1. Kongara Hanumantha Rao
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  2. S. Haseena
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Editor information

Editors and Affiliations

  1. Biotechnology Department, National Institute of Technology (NIT), Raipur, Raipur, Chhattisgarh, India

    Swasti Dhagat

  2. Biotechnology Department, National Institute of Technology (NIT), Raipur, Raipur, India

    Satya Eswari Jujjavarapu

  3. Department of Biotechnology, VFSTR University, Guntur, Andhra Pradesh, India

    N.S. Sampath Kumar

  4. Biotechnology Department, National Institute of Technology (NIT), Raipur, Raipur, India

    Chinmaya Mahapatra

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Rao, K.H., Haseena, S. (2024). Principles of Basic and Advanced Techniques Used for the Purification of Enzymes of Industrial Importance. In: Dhagat, S., Jujjavarapu, S.E., Sampath Kumar, N., Mahapatra, C. (eds) Recent Advances in Bioprocess Engineering and Bioreactor Design. Springer, Singapore. https://doi.org/10.1007/978-981-97-1451-3_8

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