Abstract
The production of biofuels from microbial sources has emerged as a promising solution to address the energy crisis and environmental concerns associated with fossil fuels. The present chapter offers a thorough overview of the most recent developments and persistent challenges in this sector. It focuses on the considerable advancements in using microorganisms, such as bacteria, yeasts, and microalgae, to produce biofuels. It explores the utilization of various feedstocks, including lignocellulosic biomass and waste materials, to generate biofuels like biodiesel, bioethanol, and biogas. The chapter also covers applications of genetic and metabolic engineering methods to improve microbial biofuel production, including raising yields and enhancing substrate usage efficiency. Despite these efforts, various obstacles stand in the way of the widespread use of microbial-based biofuel generation. Critical issues such as the availability and sustainability of feedstocks, high production costs, and effective downstream processing are also discussed in detail. It also explores the environmental implications of biofuel production and the need for developing sustainable and eco-friendly processes. Further, this chapter sheds light on the life cycle assessment approach and its assessment methods in the field of biofuel production. The national biofuel policy of India is critically analyzed in the context of the policies from different developed countries. It also emphasizes the importance of continued research and development to overcome challenges, optimize production processes, and establish microbial biofuels as a viable and sustainable alternative to conventional fuels.
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References
Ab Rasid NS, Shamjuddin A, Abdul Rahman AZ, Amin NAS (2021) Recent advances in green pre-treatment methods of lignocellulosic biomass for enhanced biofuel production. J Clean Prod 321:129038. https://doi.org/10.1016/j.jclepro.2021.129038
Agarwal A, Shaikh KM, Gharat K, Jutur PP, Pandit RA, Lali AM (2019) Investigating the modulation of metabolites under high light in mixotrophic alga Asteracys sp. using a metabolomic approach. Algal Res 43:101646. https://doi.org/10.1016/j.algal.2019.101646
Ahmed SF, Mofijur M, Chowdhury SN, Nahrin M, Rafa N, Chowdhury AT, Nuzhat S, Ong HC (2022) Pathways of lignocellulosic biomass deconstruction for biofuel and value-added products production. Fuel 318:123618. https://doi.org/10.1016/j.fuel.2022.123618
Akash B (2015) Thermochemical depolymerization of biomass. Procedia Comput Sci 52:827–834. https://doi.org/10.1016/j.procs.2015.05.139
Akinsemolu AA (2018) The role of microorganisms in achieving the sustainable development goals. J Clean Prod 182:139–155. https://doi.org/10.1016/j.jclepro.2018.02.081
Al-Wahaibi A, Osman AI, Al-Muhtaseb AH, Alqaisi O, Baawain M, Fawzy S, Rooney DW (2020) Techno-economic evaluation of biogas production from food waste via anaerobic digestion. Sci Rep 10:15719. https://doi.org/10.1038/s41598-020-72897-5
Arutselvan C, Narchonai G, Pugazhendhi A, LewisOscar F, Thajuddin N (2021) Evaluation of microalgal strains and microalgal consortium for higher lipid productivity and rich fatty acid profile towards sustainable biodiesel production. Bioresour Technol 339:125524. https://doi.org/10.1016/j.biortech.2021.125524
Aulitto M, Fusco S, Nickel DB, Bartolucci S, Contursi P, Franzén CJ (2019) Seed culture pre-adaptation of Bacillus coagulans MA-13 improves lactic acid production in simultaneous saccharification and fermentation. Biotechnol Biofuels 12:45. https://doi.org/10.1186/s13068-019-1382-2
Awasthi MK, Sar T, Gowd SC, Rajendran K, Kumar V, Sarsaiya S, Li Y, Sindhu R, Binod P, Zhang Z, Pandey A, Taherzadeh MJ (2023) A comprehensive review on thermochemical, and biochemical conversion methods of lignocellulosic biomass into valuable end product. Fuel 342:127790. https://doi.org/10.1016/j.fuel.2023.127790
Brown TR (2019) Why the cellulosic biofuels mandate fell short: a markets and policy perspective. Biofuels, Bioprod Biorefin 13:889–898. https://doi.org/10.1002/bbb.1987
Brexó RP, Sant’Ana AS (2017) Impact and significance of microbial contamination during fermentation for bioethanol production. Renew Sustain Energy Reviews 73:423–434. https://doi.org/10.1016/j.rser.2017.01.151
Candido C, Lombardi AT (2020) Mixotrophy in green microalgae grown on an organic and nutrient rich waste. World J Microbiol Biotechnol 36:20. https://doi.org/10.1007/s11274-020-2802-y
Chaurasia D, Boudh S, Singh JS, Chaturvedi P (2020) Microbes and microbial enzymes as a sustainable energy source for biofuel production. In: microbial services in restoration ecology. Elsevier, pp 207–217
Choi WJ, Chae AN, Song KG, Park J, Lee BC (2019) Effect of trophic conditions on microalga growth, nutrient removal, algal organic matter, and energy storage products in Scenedesmus (Acutodesmus) obliquus KGE-17 cultivation. Bioprocess Biosyst Eng 42:1225–1234. https://doi.org/10.1007/s00449-019-02120-x
Choi KR, Jiao S, Lee SY (2020) Metabolic engineering strategies toward production of biofuels. Curr Opin Chem Biol 59:1–14. https://doi.org/10.1016/j.cbpa.2020.02.009
Choi YJ, Lee J, Jang Y-S, Lee SY (2014) Metabolic engineering of microorganisms for the production of higher alcohols. mBio 5. https://doi.org/10.1128/mBio.01524-14
Cudjoe D, Zhu B, Wang H (2022) Towards the realization of sustainable development goals: benefits of hydrogen from biogas using food waste in China. J Clean Prod 360:132161. https://doi.org/10.1016/j.jclepro.2022.132161
Das S (2020) The national policy of biofuels of India—a perspective. Energy Policy 143:111595. https://doi.org/10.1016/j.enpol.2020.111595
Dassey AJ, Hall SG, Theegala CS (2014) An analysis of energy consumption for algal biodiesel production: comparing the literature with current estimates. Algal Res 4:89–95. https://doi.org/10.1016/j.algal.2013.12.006
Gorgich M, Mata TM, Martins AA, Branco-Vieira M, Caetano NS (2020) Comparison of different lipid extraction procedures applied to three microalgal species. Energy Rep 6:477–482. https://doi.org/10.1016/j.egyr.2019.09.011
Hao H, Liu Z, Zhao F, Ren J, Chang S, Rong K, Du J (2018) Biofuel for vehicle use in China: current status, future potential and policy implications. Renew Sustain Energy Rev 82:645–653. https://doi.org/10.1016/j.rser.2017.09.045
Holwerda EK, Worthen RS, Kothari N, Lasky RC, Davison BH, Fu C, Wang Z-Y, Dixon RA, Biswal AK, Mohnen D, Nelson RS, Baxter HL, Mazarei M, Stewart CN, Muchero W, Tuskan GA, Cai CM, Gjersing EE, Davis MF, Himmel ME, Wyman CE, Gilna P, Lynd LR (2019) Multiple levers for overcoming the recalcitrance of lignocellulosic biomass. Biotechnol Biofuels 12:15. https://doi.org/10.1186/s13068-019-1353-7
Hu H, Li J-Y, Zhai S-W, Wu D-D, Zhu S-G, Zeng RJ (2020) Effect of inorganic carbon limitation on the conversion of organic carbon to total fatty acids by Monodus subterraneus. Sci Total Environ 737:140275. https://doi.org/10.1016/j.scitotenv.2020.140275
Hwangbo M, Chu K-H (2020) Recent advances in production and extraction of bacterial lipids for biofuel production. Sci Total Environ 734:139420. https://doi.org/10.1016/j.scitotenv.2020.139420
Joshi S, Mishra S (2022) Recent advances in biofuel production through metabolic engineering. Bioresour Technol 352:127037. https://doi.org/10.1016/j.biortech.2022.127037
Joshi G, Pandey JK, Rana S, Rawat DS (2017) Challenges and opportunities for the application of biofuel. Renew Sustain Energy Rev 79:850–866. https://doi.org/10.1016/j.rser.2017.05.185
Joshi A, Verma KK, Rajput VD, Arora J (2023) Metabolic engineering of microorganisms in advancing biofuel production. In: Advances in lignocellulosic biofuel production systems. Elsevier, pp 181–202
Khoo KS, Tan X, Ooi CW, Chew KW, Leong WH, Chai YH, Ho S-H, Show PL (2021) How does ionic liquid play a role in sustainability of biomass processing? J Clean Prod 284:124772. https://doi.org/10.1016/j.jclepro.2020.124772
Khoo KS, Ahmad I, Chew KW, Iwamoto K, Bhatnagar A, Show PL (2023) Enhanced microalgal lipid production for biofuel using different strategies including genetic modification of microalgae: a review. Prog Energy Combust Sci 96:101071. https://doi.org/10.1016/j.pecs.2023.101071
Li T, Yang F, Xu J, Wu H, Mo J, Dai L, Xiang W (2020) Evaluating differences in growth, photosynthetic efficiency, and transcriptome of Asterarcys sp. SCS-1881 under autotrophic, mixotrophic, and heterotrophic culturing conditions. Algal Res 45:101753. https://doi.org/10.1016/j.algal.2019.101753
Lin L, Cunshan Z, Vittayapadung S, Xiangqian S, Mingdong D (2011) Opportunities and challenges for biodiesel fuel. Appl Energy 88:1020–1031. https://doi.org/10.1016/j.apenergy.2010.09.029
López-Fernández J, Dolors Benaiges M, Valero F (2021) Second- and third-generation biodiesel production with immobilised recombinant Rhizopus oryzae lipase: influence of the support, substrate acidity and bioprocess scale-up. Bioresour Technol 334:125233. https://doi.org/10.1016/j.biortech.2021.125233
Luthra S, Kumar S, Garg D, Haleem A (2015) Barriers to renewable/sustainable energy technologies adoption: Indian perspective. Renew Sustain Energy Rev 41:762–776. https://doi.org/10.1016/j.rser.2014.08.077
Manzoor M, Ahmad Q, Aslam A, Jabeen F, Rasul A, Schenk PM, Qazi JI (2020) Mixotrophic cultivation of Scenedesmus dimorphus in sugarcane bagasse hydrolysate. Environ Prog Sustain Energy 39. https://doi.org/10.1002/ep.13334
Menegol T, Romero-Villegas GI, López-Rodríguez M, Navarro-López E, López-Rosales L, Chisti Y, Cerón-García MC, Molina-Grima E (2019) Mixotrophic production of polyunsaturated fatty acids and carotenoids by the microalga Nannochloropsis gaditana. J Appl Phycol 31:2823–2832. https://doi.org/10.1007/s10811-019-01828-3
Merlin Christy P, Gopinath LR, Divya D (2014) A review on anaerobic decomposition and enhancement of biogas production through enzymes and microorganisms. Renew Sustain Energy Rev 34:167–173. https://doi.org/10.1016/j.rser.2014.03.010
Novia N, Hasanudin H, Hermansyah H, Fudholi A, Pareek VK (2023) Recent advances in CFD modeling of bioethanol production processes. Renew Sustain Energy Rev 183:113522. https://doi.org/10.1016/j.rser.2023.113522
Pang N, Gu X, Fu X, Chen S (2019) Effects of gluconate on biomass improvement and light stress tolerance of Haematococcus pluvialis in mixotrophic culture. Algal Res 43:101647. https://doi.org/10.1016/j.algal.2019.101647
Pardo-Cárdenas Y, Herrera-Orozco I, González-Delgado Á-D, Kafarov V (2013) Environmental assessment of microalgae biodiesel production in Colombia: comparison of three oil extraction systems. CT&F Ciencia Tecnol Fut 5:85–100
Patel A, Matsakas L, Rova U, Christakopoulos P (2018) Heterotrophic cultivation of Auxenochlorella protothecoides using forest biomass as a feedstock for sustainable biodiesel production. Biotechnol Biofuels 11:169. https://doi.org/10.1186/s13068-018-1173-1
Poddar N, Sen R, Martin GJO (2018) Glycerol and nitrate utilisation by marine microalgae nannochloropsis salina and Chlorella sp. and associated bacteria during mixotrophic and heterotrophic growth. Algal Res 33:298–309. https://doi.org/10.1016/j.algal.2018.06.002
Popp J, Lakner Z, Harangi-Rákos M, Fári M (2014) The effect of bioenergy expansion: food, energy, and environment. Renew Sustain Energy Rev 32:559–578. https://doi.org/10.1016/j.rser.2014.01.056
Pramanik SK, Suja FB, Zain SM, Pramanik BK (2019) The anaerobic digestion process of biogas production from food waste: prospects and constraints. Bioresour Technol Rep 8:100310. https://doi.org/10.1016/j.biteb.2019.100310
Prasad RK, Chatterjee S, Mazumder PB, Gupta SK, Sharma S, Vairale MG, Datta S, Dwivedi SK, Gupta DK (2019) Bioethanol production from waste lignocelluloses: a review on microbial degradation potential. Chemosphere 231:588–606. https://doi.org/10.1016/j.chemosphere.2019.05.142
Preethi MG, Kumar G, Karthikeyan OP, Varjani S, J. RB, (2021) Lignocellulosic biomass as an optimistic feedstock for the production of biofuels as valuable energy source: techno-economic analysis, environmental impact analysis, breakthrough and perspectives. Environ Technol Innov 24:102080. https://doi.org/10.1016/j.eti.2021.102080
Raja Ram N, Nikhil GN (2022) A critical review on sustainable biogas production with focus on microbial-substrate interactions: bottlenecks and breakthroughs. Bioresour Technol Rep 19:101170. https://doi.org/10.1016/j.biteb.2022.101170
Rastogi M, Shrivastava S (2017) Recent advances in second generation bioethanol production: an insight to pretreatment, saccharification and fermentation processes. Renew Sustain Energy Rev 80:330–340
Rodionova MV, Bozieva AM, Zharmukhamedov SK, Leong YK, Chi-Wei Lan J, Veziroglu A, Veziroglu TN, Tomo T, Chang J-S, Allakhverdiev SI (2022) A comprehensive review on lignocellulosic biomass biorefinery for sustainable biofuel production. Int J Hydrogen Energy 47:1481–1498. https://doi.org/10.1016/j.ijhydene.2021.10.122
Saranya G, Ramachandra TV (2020) Life cycle assessment of biodiesel from estuarine microalgae. Energy Convers Manag X 8:100065. https://doi.org/10.1016/j.ecmx.2020.100065
Singh S, Kumar A, Sivakumar N, Verma JP (2022) Deconstruction of lignocellulosic biomass for bioethanol production: Recent advances and future prospects. Fuel 327:125109. https://doi.org/10.1016/j.fuel.2022.125109
Sorda G, Banse M, Kemfert C (2010) An overview of biofuel policies across the world. Energy Policy 38:6977–6988. https://doi.org/10.1016/j.enpol.2010.06.066
Tannous S, Manneh R, Harajli H, El Zakhem H (2018) Comparative cradle-to-grave life cycle assessment of traditional grid-connected and solar stand-alone street light systems: a case study for rural areas in Lebanon. J Clean Prod 186:963–977. https://doi.org/10.1016/j.jclepro.2018.03.155
Tsapekos P, Khoshnevisan B, Zhu X, Treu L, Alfaro N, Kougias PG, Angelidaki I (2022) Lab- and pilot-scale anaerobic digestion of municipal bio-waste and potential of digestate for biogas upgrading sustained by microbial analysis. Renew Energy 201:344–353. https://doi.org/10.1016/j.renene.2022.10.116
Vasistha S, Khanra A, Clifford M, Rai MP (2021) Current advances in microalgae harvesting and lipid extraction processes for improved biodiesel production: a review. Renew Sustain Energy Rev 137:110498. https://doi.org/10.1016/j.rser.2020.110498
Vijayakumar P, Ayyadurai S, Arunachalam KD, Mishra G, Chen W-H, Juan JC, Naqvi SR (2022) Current technologies of biochemical conversion of food waste into biogas production: a review. Fuel 323:124321. https://doi.org/10.1016/j.fuel.2022.124321
Wang H, Zhang Y, Zhou W, Noppol L, Liu T (2018) Mechanism and enhancement of lipid accumulation in filamentous oleaginous microalgae Tribonema minus under heterotrophic condition. Biotechnol Biofuels 11:328. https://doi.org/10.1186/s13068-018-1329-z
Wang X, Zhang M-M, Sun Z, Liu S-F, Qin Z-H, Mou J-H, Zhou Z-G, Lin CSK (2020) Sustainable lipid and lutein production from Chlorella mixotrophic fermentation by food waste hydrolysate. J Hazard Mater 400:123258. https://doi.org/10.1016/j.jhazmat.2020.123258
Wang H, Peng X, Zhang H, Yang S, Li H (2021) Microorganisms-promoted biodiesel production from biomass: a review. Energy Convers Manag X 12:100137. https://doi.org/10.1016/j.ecmx.2021.100137
Wang N, Xu B, Wang X, Lang J, Zhang H (2022) Chemical and structural elucidation of lignin and cellulose isolated using DES from bagasse based on alkaline and hydrothermal pretreatment. Polymers (basel) 14:2756. https://doi.org/10.3390/polym14142756
Zhang Q, De Oliveira VK, Royer S, Jérôme F (2012) Deep eutectic solvents: syntheses, properties and applications. Chem Soc Rev 41:7108. https://doi.org/10.1039/c2cs35178a
Zhang C, Sun Y, Cao T, Wang W, Huo S, Liu Z-H (2022) Influence of organic load on biogas production and response of microbial community in anaerobic digestion of food waste. Int J Hydrogen Energy 47:32849–32860. https://doi.org/10.1016/j.ijhydene.2022.07.187
Zhao Z, Ma S, Li A, Liu P, Wang M (2016) Effects of trophic modes, carbon sources, and salinity on the cell growth and lipid accumulation of tropic ocean oilgae strain Desmodesmus sp. WC08. Appl Biochem Biotechnol 180:452–463. https://doi.org/10.1007/s12010-016-2109-5
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Sandeep, B., Baranwal, U., Mandpe, A. (2024). Biofuel Production from Microbial Sources: Advances and Challenges. In: Bala, K., Ghosh, T., Kumar, V., Sangwan, P. (eds) Harnessing Microbial Potential for Multifarious Applications. Energy, Environment, and Sustainability. Springer, Singapore. https://doi.org/10.1007/978-981-97-1152-9_13
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