Abstract
Contamination due to the spillage of petroleum or petroleum-derived hydrocarbons on different ecosystems has become critical issues to environment and living things. The use of native bacteria as an environmental friendly treatment of hydrocarbons pollution is very promising. The objective of the study is to identify the native hydrocarbon-degraders isolated from oil refinery. Bacteria from crude oil were isolated and cultured in enrichment media. The isolated strains were then cultured in different oil concentration. The isolated bacterial strains were Gram-stained and further identified via 16S rRNA sequencing. The resultant sequences were then aligned with the available online database for identification of the bacteria. The isolated bacteria belonged to families of Pseudomonadaceae and Moraxellaceae (Acinetobacter genus). The bacteria show high degradation of hydrocarbon and can be used to remediate polluted soils in tropical environment.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Gomes NCM, Kosheleva IA, Abraham W-R, Smalla K (2005) Effects of the inoculant strain Pseudomonas putida KT2442 (pNF142) and of naphthalene contamination on the soil bacterial community. FEMS Microbiol Ecol 54:21–33
Lal B, Khanna S (1996) Degradation of crude oil by Acinetobacter calcoaceticus and Alcaligenes odorans. J Appl Microbiol 81:355–362
Rahman KSM, Thahira-Rahman J, Kourkoutas Y, Petsas I, Marchant R, Banat IM (2003) bioremediation of n-alkanes in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients. Bioresour Technol 90(2):159–168
Thapa B, Kumar A, Ghimire A (2012) A review on bioremediation of petroleum hydrocarbon contaminants in soil. Kathmandu University Journal of Science, Engineering and Technology, p 8
Wang J, Zhang Z, Su Y, He W, He F, Song H (2008) Phytoremediation of petroleum polluted soil. Petroleum Science, p 5
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media Singapore
About this paper
Cite this paper
Alavijeh, P.K., Halimoon, N., Zakaria, M.P., Johari, W.L.W., Karimi, B. (2014). Crude Oil Biodegradation Using Isolated Bacteria from Oil Polluted Soil. In: Aris, A., Tengku Ismail, T., Harun, R., Abdullah, A., Ishak, M. (eds) From Sources to Solution. Springer, Singapore. https://doi.org/10.1007/978-981-4560-70-2_24
Download citation
DOI: https://doi.org/10.1007/978-981-4560-70-2_24
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-4560-69-6
Online ISBN: 978-981-4560-70-2
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)