Abstract
In this chapter, the fabrication of robust biomolecule microarrays by reactive microcontact printing (μCP) on spin-coated thin films of poly(N-hydroxysuccinimidyl-methacrylate) (PNHSMA) on oxidized silicon and glass is described. The approach combines the advantages of activated polymer thin films as coupling layers, characterized by high reactivity and high molecular loading (see Chaps. 3 and 4), with the versatility and flexibility of soft lithography.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Singhvi R, Kumar A, Lopez GP, Stephanopoulos GN, Wang DI, Whitesides GM (1994) Engineering cell-shape and function. Science 264:696–698
MacBeath G, Schreiber SL (2000) Printing proteins as microarrays for high-throughput function determination. Science 289:1760–1763
Brack HP, Padeste C, Slaski M, Alkan S, Solak HH (2004) Preparation of micro- and nanopatterns of polymer chains grafted onto flexible polymer substrates. J Am Chem Soc 126:1004–1005
Lussi JW, Michel R, Reviakine I, Falconnet D, Goessl A, Csucs G, Hubbell JA, Textor M (2004) A novel generic platform for chemical patterning of surface. Prog Surf Sci 76:55–69
Wang C, Zhang Y (2005) Protein micropatterning via self-assembly of nanoparticles. Adv Mater 17:150–153
Kumar A, Whitesides GM (2002) Features of gold having micrometer to centimeter dimensions can be formed through a combination of stamping with an elastomeric stamp and an alkanethiol ink followed by chemical etching. Appl Phys Lett 63:2002–2004
Crooks RM, Ricco AJ (1998) New organic materials suitable for use in chemical sensor arrays. Acc Chem Res 31:219–227
Quake SR, Scherer A (2000) From micro- to nanofabrication with soft materials. Science 290:1536–1540
Renault JP, Bernard A, Juncker D, Michel B, Bosshare HR, Delamarche E (2002) Fabricating microarrays of functional proteins using affinity contact printing. Angew Chem Int Ed 41:2320–2323
Anzai J, Kobayashi Y, Nakamura N, Nishimura M, Hoshi T (1999) Layer-by-layer construction of multilayer thin films composed of avidin and biotin-labeled poly(amine)s. Langmuir 15:221–226
Pathak S, Singh AK, McElhanon JR, Dentinger PM (2004) Dendrimer-activated surfaces for high density and high activity protein chip applications. Langmuir 20:6075–6079
Degenhart GH, Dordi B, Schönherr H, Vancso GJ (2004) Micro- and nanofabrication of robust reactive arrays based on the covalent coupling of dendrimers to activated monolayers. Langmuir 20:6216–6264
Xia YN, Mrksich M, Kim E, Whitesides GM (1995) Microcontact printing of octadecylsiloxane on the surface of silicon dioxide and its application in microfabrication. J Am Chem Soc 117:9576–9577
Knoll W, Matsuzawa M, Offenhäusser A, Rühe J (1996) Tailoring of surfaces with ultrathin layers for controlled binding of biopolymers and adhesion and guidance of cells. Isr J Chem 36:357–369
Wells M, Crooks RM (1996) Interactions between organized, surface-confined monolayers and vapor-phase probe molecules.10. Preparation and properties of chemically sensitive dendrimer surfaces. J Am Chem Soc 118:3988–3989
Langer R, Vacanti JP (1993) Tissue engineering. Science 260:920–926
Bruening ML, Zhou Y, Aguilar G, Agee R, Bergbreiter DE, Crooks RM (1997) Synthesis and characterization of surface-grafted, hyperbranched polymer films containing fluorescent, hydrophobic, ion-binding, biocompatible, and electroactive groups. Langmuir 13:770–778
Malmqvist M, Karlsson R (1997) Biomolecular interaction analysis: affinity biosensor technologies for functional analysis of proteins. Curr Opin Chem Bio 1:378–383
Niemeyer CM, Blohm D (1999) DNA microarrays. Angew Chem Int Ed 38:2865–2869
Rowan B, Wheeler MA, Crooks RM (2002) Patterning bacteria within hyperbranched polymer film templates. Langmuir 18:9914–9917
Lahann J, Balcells M, Rodon T, Lee J, Choi IS, Jensen KF, Langer R (2002) Reactive polymer coatings: A platform for patterning proteins and mammalian cells onto a broad range of materials. Langmuir 18:3632–3638
Zhou X, Wu L, Zhou J (2004) Fabrication of DNA microarrays on nanoengineered polymeric ultrathin film prepared by self-assembly of polyelectrolyte multilayers. Langmuir 20:8877–8885
Zhang Z, Knoll W, Foerch R, Holcomb R, Roitman D (2005) DNA hybridization on plasma-polymerized allylamine. Macromolecules 38:1271–1276
Jérôme C, Gabriel S, Voccia S, Detrembleur C, Ignatova M, Gouttebaron R, Jérôme R (2003) Preparation of reactive surfaces by electrografting. Chem Commun 19:2500–2501
Yu F, Persson B, Löfås S, Knoll W (2004) Attomolar sensitivity in bioassays based on surface plasmon fluorescence spectroscopy. J Am Chem Soc 126:8902–8903
Frutos AG, Brocknam JM, Corn RM (2000) Reversible protection and reactive patterning of amine-and hydroxyl-terminated self-assembled monolayers on gold surfaces for the fabrication of biopolymer arrays. Langmuir 16:2192–2197
Groll J, Ameringer T, Spatz J, Möller M (2005) Ultrathin coatings from isocyanate-terminated star PEG prepolymers: Layer formation and characterization. Langmuir 21:1991–1999
Gong P, Grainger DW (2004) Comparison of DNA immobilization efficiency on new and regenerated commercial amine-reactive polymer microarray surfaces. Surf Sci 570:67–77
Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE (1997) Geometric control of cell life and death. Science 276:1425–1428
Prime KL, Whitesides GM (1993) Adsorption of proteins onto surfaces containing end-attached oligo(ethylene oxide): a model system using self-assembled monolayers. J Am Chem Soc 115:10714–10721
Kumar A, Whitesides GM (1993) Features of gold having micrometer to centimeter dimensions can be formed through a combination of stamping with an elastomeric stamp and an alkanethiol “ink” followed by chemical etching. Appl Phys Lett 63:2002–2004
Reilman RF, Msezane A, Manson ST (1976) Relative intensities in photoelectron-spectroscopy of atoms and molecules. J Electron Spectrosc 8:389–394
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2021 Shanghai Jiao Tong University Press
About this chapter
Cite this chapter
Feng, C., Dou, X., Xu, Y. (2021). Fabrication of Robust Biomolecular Patterns by Reactive Microcontact Printing on NHS Ester Containing Polymer Films. In: Microfabrication of Stimuli-Responsive Polymers. Springer, Singapore. https://doi.org/10.1007/978-981-33-6869-9_6
Download citation
DOI: https://doi.org/10.1007/978-981-33-6869-9_6
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-33-6868-2
Online ISBN: 978-981-33-6869-9
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)